Integrating genomics in pigeonpea hybrid breeding
Material type:
TextPublication details: 2010Description: 1 pageSummary: For a quantum jump in yield, the development of hybrids using male- sterility system was planned and the world?s first commercial CMS-based pigeonpea hybrid ICPH 2671 was released by ICRISAT in collaboration with a private company Pravardhan Seeds, that recorded 25-30% yield advantage in farmers? fields. Since this hybrid technology is new, a hybrid breeding system needs to be versatile to adapt to different agro ecological zones and cropping systems. A high level of genetic purity is essential in maintaining the necessary level of heterosis observed in this crop. After screening 159 A- (male-sterile), B- (maintainer) and R- (fertility restorer) lines with 148 simple sequence repeat (SSR) markers, 41 markers showed polymorphism with 2 to 6 (average 2.6) alleles and 0.01 to 0.81 (average 0.34) polymorphism information content value (PIC). Of these polymorphic markers, two SSR markers (CCB4 and CCttc006) were found suitable for purity assessment of hybrid seeds and for checking heterogeneity in the parental lines of ICPH 2438 hybrid. In parallel four F2 populations segregating for fertility restorer (Rf) genes were developed, traditional phenotypic analysis shows involvement of two to three dominant genes in restoring fertility. Parental genotypes of these mapping populations were also screened for polymorphism with 3072 SSRs for genetic mapping of Rf genes. With an objective of understanding the basis of CMS in pigeonpea, efforts have also been initiated to sequence the mitochondrial genomes of ICPA 2039, ICPB 2039, ICPH 2433 and ICPW 29 by using 454/FLX sequencing technology.
| Item type | Current library | Collection | Call number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|
| Conference proceedings | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Staff Publications Collection | CIS-6169 (Browse shelf(Opens below)) | Available |
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For a quantum jump in yield, the development of hybrids using male- sterility system was planned and the world?s first commercial CMS-based pigeonpea hybrid ICPH 2671 was released by ICRISAT in collaboration with a private company Pravardhan Seeds, that recorded 25-30% yield advantage in farmers? fields. Since this hybrid technology is new, a hybrid breeding system needs to be versatile to adapt to different agro ecological zones and cropping systems. A high level of genetic purity is essential in maintaining the necessary level of heterosis observed in this crop. After screening 159 A- (male-sterile), B- (maintainer) and R- (fertility restorer) lines with 148 simple sequence repeat (SSR) markers, 41 markers showed polymorphism with 2 to 6 (average 2.6) alleles and 0.01 to 0.81 (average 0.34) polymorphism information content value (PIC). Of these polymorphic markers, two SSR markers (CCB4 and CCttc006) were found suitable for purity assessment of hybrid seeds and for checking heterogeneity in the parental lines of ICPH 2438 hybrid. In parallel four F2 populations segregating for fertility restorer (Rf) genes were developed, traditional phenotypic analysis shows involvement of two to three dominant genes in restoring fertility. Parental genotypes of these mapping populations were also screened for polymorphism with 3072 SSRs for genetic mapping of Rf genes. With an objective of understanding the basis of CMS in pigeonpea, efforts have also been initiated to sequence the mitochondrial genomes of ICPA 2039, ICPB 2039, ICPH 2433 and ICPW 29 by using 454/FLX sequencing technology.
English
Lucia Segura
CIMMYT Staff Publications Collection
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