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Large-scale screening for drought tolerance at vegetative stage as revealed by biomass measured under water-stressed and well-watered environments, compared with other selection criteria

By: Contributor(s): Material type: TextTextLanguage: English Publication details: 2009. China : SAGC. Description: 2 pagesSubject(s): Summary: A total of 550 lines collected from breeding programs across the world were evaluated under both well-watered and water-stressed environments (each with two replications) during the 2008 dry season at Tlaltizapan (State of Morelos, Mexico). Water stress at vegetative and flowering stages was imposed by stopping irrigation for four weeks at 6-leaf stage and 2 weeks prior to anthesis, respectively. Vegetative stage screening was based on multiple measurements of biomass before and after the drought stress was applied using the normalized difference vegetation index (NDVI) measured with a portable spectroradiometer (GreenSeeker). Other drought tolerancerelated selection criteria evaluated include anthesis-silking interval (ASI), leaf senescence, chlorophyll content, root capacitance, final grain yield and drought tolerance index (DTI, final grain yield under stressed condition compared with the well-watered). Under both well-watered and water-stressed conditions, NDVI was significantly positively correlated with chlorophyll content and final grain yield, while negatively correlated with leaf senescence. However, there was no significant correlation of NDVI with ASI or root capacitance. The relative NDVI measurements for the water-stressed environment compared to the well-watered environment did not show a significant relationship with any other selection criteria. In addition, none of the selection criteria were significantly correlated with DTI. Selection index was constructed for drought tolerance based on multiple traits. The 45 lines with the best level of drought tolerance produced an average of more than 700 kernels per plot. All the tested lines have been genotyped using two single nucleotide polymorphism chips each containing 1536 markers. Linkage and linkage disequilibrium mapping for drought tolerance has been carried out and are presented by Lu et al and Hao et al (this conference).
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Conference proceedings CIMMYT Knowledge Center: John Woolston Library CIMMYT Staff Publications Collection CIS-5728 (Browse shelf(Opens below)) Available
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Abstract only

A total of 550 lines collected from breeding programs across the world were evaluated under both well-watered and water-stressed environments (each with two replications) during the 2008 dry season at Tlaltizapan (State of Morelos, Mexico). Water stress at vegetative and flowering stages was imposed by stopping irrigation for four weeks at 6-leaf stage and 2 weeks prior to anthesis, respectively. Vegetative stage screening was based on multiple measurements of biomass before and after the drought stress was applied using the normalized difference vegetation index (NDVI) measured with a portable spectroradiometer (GreenSeeker). Other drought tolerancerelated selection criteria evaluated include anthesis-silking interval (ASI), leaf senescence, chlorophyll content, root capacitance, final grain yield and drought tolerance index (DTI, final grain yield under stressed condition compared with the well-watered). Under both well-watered and water-stressed conditions, NDVI was significantly positively correlated with chlorophyll content and final grain yield, while negatively correlated with leaf senescence. However, there was no significant correlation of NDVI with ASI or root capacitance. The relative NDVI measurements for the water-stressed environment compared to the well-watered environment did not show a significant relationship with any other selection criteria. In addition, none of the selection criteria were significantly correlated with DTI. Selection index was constructed for drought tolerance based on multiple traits. The 45 lines with the best level of drought tolerance produced an average of more than 700 kernels per plot. All the tested lines have been genotyped using two single nucleotide polymorphism chips each containing 1536 markers. Linkage and linkage disequilibrium mapping for drought tolerance has been carried out and are presented by Lu et al and Hao et al (this conference).

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