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Chromosome doubling in maize anther culture by Colchicine and Pronamide treatment

By: Sripichitt, P | Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico, DF (Mexico) | Proceedings of the Asian Regional Maize Workshop Bangkok (Thailand) 5-8 Aug 2002.
Contributor(s): Saengduen, N [coaut.] | Srinivasan, G.|Zaidi, P.H.|Prasanna, B.M.|Gonzalez, F.|Lesnick, K [eds.] | Supapanyapong, P [coaut.] | Wongyai, W [coaut.].
Material type: materialTypeLabelBookAnalytics: Show analyticsPublisher: Mexico, DF (Mexico) CIMMYT : 2004Description: p. 109-118.ISBN: 970-648-116-8.Subject(s): Androgenesis | Anther culture | Chromosomes | Colchicine | Maize | Treatment date | CIMMYT | Genotypes AGROVOC | Hybrids AGROVOCSummary: The frequency of spontaneous chromosome doubling occurring during androgenesis of maize is relatively low. Attempts were made in this study to determine efficient methods of artificial chromosome doubling to increase the number of doubled haploid plants regenerated from cultured anthers of maize. Colchicine and pronamide were used as chromosome doubling agents. Anthers of the FI hybrid between Ki3 and M24 were cultured on induction medium supplemented with colchicine at the concentrations of 0, 125, 250, 500 and 1,000 mg/1 and pronamide at the concentrations of 0. 2.5, 5, 10, 20 and 40 mM for 0, 3, 5 and 7 days. After the treatment for various durations, anthers were subcultured onto the same induction medium without colchicine and pronamide until the complete period of induction (3 weeks). Anthers were then transferred to regeneration medium for embryo-like structure (ES) formation and plant regeneration. Chromosomes from root tip of each regenerated plant (RP) were counted by squash method with 1% aceto-carmine staining. It was found that pronamide at the concentration of 40 mM applied to anthers for 7 days gave the highest frequency of ES formation (15.55 ES/100 anthers) and plant regeneration (5.78 RP/100 anthers). Spontaneous chromosome doubling occurred at the rate of 10.3% when the anthers were cultured on induction medium without doubling agent. Colchicine at the concentration of 125 mg/1 applied to anthers for 3 days induced the highest percentage of chromosome doubling (35.3%) followed by pronamide at the concentration of 20 µM gave to anthers for 3 days, which caused chromosome doubling at the rate of 34.8%.Collection: CIMMYT Publications Collection
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The frequency of spontaneous chromosome doubling occurring during androgenesis of maize is relatively low. Attempts were made in this study to determine efficient methods of artificial chromosome doubling to increase the number of doubled haploid plants regenerated from cultured anthers of maize. Colchicine and pronamide were used as chromosome doubling agents. Anthers of the FI hybrid between Ki3 and M24 were cultured on induction medium supplemented with colchicine at the concentrations of 0, 125, 250, 500 and 1,000 mg/1 and pronamide at the concentrations of 0. 2.5, 5, 10, 20 and 40 mM for 0, 3, 5 and 7 days. After the treatment for various durations, anthers were subcultured onto the same induction medium without colchicine and pronamide until the complete period of induction (3 weeks). Anthers were then transferred to regeneration medium for embryo-like structure (ES) formation and plant regeneration. Chromosomes from root tip of each regenerated plant (RP) were counted by squash method with 1% aceto-carmine staining. It was found that pronamide at the concentration of 40 mM applied to anthers for 7 days gave the highest frequency of ES formation (15.55 ES/100 anthers) and plant regeneration (5.78 RP/100 anthers). Spontaneous chromosome doubling occurred at the rate of 10.3% when the anthers were cultured on induction medium without doubling agent. Colchicine at the concentration of 125 mg/1 applied to anthers for 3 days induced the highest percentage of chromosome doubling (35.3%) followed by pronamide at the concentration of 20 µM gave to anthers for 3 days, which caused chromosome doubling at the rate of 34.8%.

English

0501|AGRIS 0501|AL-Maize Program

Juan Carlos Mendieta

CIMMYT Publications Collection

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