Normal view MARC view ISBD view

Assessment of genetic similarities among maize inbread lines using SSR markers

By: Yuan, L | Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico, DF (Mexico) | Proceedings of the Asian Regional Maize Workshop Bangkok (Thailand) 5-8 Aug 2002.
Contributor(s): Fu, J [coaut.] | Li, M [coaut.] | Li, X [coaut.] | Srinivasan, G.|Zaidi, P.H.|Prasanna, B.M.|Gonzalez, F.|Lesnick, K [eds.] | Warburton, M [coaut.] | Zhang, S [coaut.].
Material type: materialTypeLabelBookAnalytics: Show analyticsPublisher: Mexico, DF (Mexico) CIMMYT : 2004Description: p. 50-58.ISBN: 970-648-116-8.Subject(s): Genetic markers AGROVOC | Heterotis | Inbred lines | Maize | Plant protection | Polyacrylamide | Polymorphism | CIMMYT | Genetic resources | Hybrids AGROVOC | Plant breeding AGROVOCSummary: Knowledge of genetic diversity and of relationships among the elite breeding materials can have a significant impact on maize breeding. SSR markers have proven to be reliable and highly informative for assessing genetic diversity in maize. In the present study, 134 maize inbred lines from China and CIMMYT were assayed for polymorphism at 51 SSR marker loci using non-denaturing polyacrylamide gels. A total of 222 alleles were detected among these lines with an average of 4.4 alleles per locus. The values of polymorphic information content (PIC) for these loci ranged from 0.14 to 0.87, with an average of 0.56. The relationships among the lines revealed by genetic similarity (GS) coefficient were consistent with known pedigrees. The dendrogram based on the similarity matrix using UPGMA algorithm delineated the above lines into nine clusters. Most of the CIMMYT lines representing subtropical and tropical germplasm were assigned into one major cluster, and the lines from the Chinese germplasm were placed into the remaining eight clusters (Sipingtou, Luda Red Cob, Lancaster, BSSS, PA, PB, Zi330, and Zhong). The results have provided useful information for assigning lines to heterotic groups, planning crosses for hybrid and line development, and choosing parents for breeding program.Collection: CIMMYT Staff Publications Collection
Tags from this library: No tags from this library for this title. Log in to add tags.
    average rating: 0.0 (0 votes)
Item type Current location Collection Call number Copy number Status Date due Barcode Item holds
Conference proceedings CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

CIMMYT Staff Publications Collection CIS-4289 (Browse shelf) 1 Available 630603
Total holds: 0

Knowledge of genetic diversity and of relationships among the elite breeding materials can have a significant impact on maize breeding. SSR markers have proven to be reliable and highly informative for assessing genetic diversity in maize. In the present study, 134 maize inbred lines from China and CIMMYT were assayed for polymorphism at 51 SSR marker loci using non-denaturing polyacrylamide gels. A total of 222 alleles were detected among these lines with an average of 4.4 alleles per locus. The values of polymorphic information content (PIC) for these loci ranged from 0.14 to 0.87, with an average of 0.56. The relationships among the lines revealed by genetic similarity (GS) coefficient were consistent with known pedigrees. The dendrogram based on the similarity matrix using UPGMA algorithm delineated the above lines into nine clusters. Most of the CIMMYT lines representing subtropical and tropical germplasm were assigned into one major cluster, and the lines from the Chinese germplasm were placed into the remaining eight clusters (Sipingtou, Luda Red Cob, Lancaster, BSSS, PA, PB, Zi330, and Zhong). The results have provided useful information for assigning lines to heterotic groups, planning crosses for hybrid and line development, and choosing parents for breeding program.

English

0501|AGRIS 0501|AL-Maize Program

Juan Carlos Mendieta

CIMMYT Staff Publications Collection

There are no comments for this item.

Log in to your account to post a comment.
baner

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2015. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
Monday –Friday 9:00 am. 17:00 pm. If you have any question, please contact us at CIMMYT-Knowledge-Center@cgiar.org

Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT) © Copyright 2015. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
Lunes –Viernes 9:00 am. 17:00 pm. Si tiene cualquier pregunta, contáctenos a CIMMYT-Knowledge-Center@cgiar.org