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Expression of a synthetic porcine transgene increases lysine levels in maize kernels

By: Bicar, E | Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico DF (Mexico) | Arnel R. Hallauer International Symposium on Plant Breeding Mexico, D.F. (Mexico) 17-22 Aug 2003.
Contributor(s): Lee, M [coaut.] | Scott, P [coaut.].
Material type: materialTypeLabelBookAnalytics: Show analyticsPublisher: Mexico, DF (Mexico) CIMMYT : 2003Description: p. 167.Subject(s): Amino acids | Cereals | Genomes | Kernels AGROVOC | Lysine | Maize | Nutritive value | Technology | transgenicsDDC classification: 631.53 Summary: Cereal grains are major sources of nutrition for humans and other anima1s. The major cereals are defident in one or more of the essential amino acids such as lysine and tryptophan and, therefore, do not provide a well-balanced diet. These defidendes limit the nutritional value of the grain. Recent advances in genetic engineering and improvements in transformation technology have provided new opportunities to modify the nutritional composition of the maize grain. In an effort to elevate the amount of lysine in maize grain, a codon-adjusted version of a gene encoding the porcine milk protein a-lactalbumin was synthesized and used to transform maize. The goals in this study were: (1) to determine if the synthetic porcie a-La transgenes were integrated and functional in the maize genome; (2) to characterize the inheritance and expression patterns of the transgenes; and (3) to determine the leve1s of the a-La protein and the correlated changes in amino acid composition of transformed maize kemels.Collection: CIMMYT Publications Collection
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Conference proceedings CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

CIMMYT Publications Collection 631.53 BOO (Browse shelf) 1 Available 2A632399
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Cereal grains are major sources of nutrition for humans and other anima1s. The major cereals are defident in one or more of the essential amino acids such as lysine and tryptophan and, therefore, do not provide a well-balanced diet. These defidendes limit the nutritional value of the grain. Recent advances in genetic engineering and improvements in transformation technology have provided new opportunities to modify the nutritional composition of the maize grain. In an effort to elevate the amount of lysine in maize grain, a codon-adjusted version of a gene encoding the porcine milk protein a-lactalbumin was synthesized and used to transform maize. The goals in this study were: (1) to determine if the synthetic porcie a-La transgenes were integrated and functional in the maize genome; (2) to characterize the inheritance and expression patterns of the transgenes; and (3) to determine the leve1s of the a-La protein and the correlated changes in amino acid composition of transformed maize kemels.

English

0309|AGRIS 0301|AL-Maize Program

Juan Carlos Mendieta

CIMMYT Publications Collection

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