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Session 5. Paper 2. Morphological and molecular variations within Heterodera filipjevi populations from Iran compared to other populations

By: Toumi, F.
Contributor(s): Tanha Maafi, Z | Waeyenberge, L | Viaene, N | Dababat, A.A | Nicol, J.M | Ogbonnaya, F.C | Moens, M.
Material type: materialTypeLabelBookAnalytics: Show analyticsPublisher: Agadir, Morocco : INRA : CIMMYT : MFAL, 2017Subject(s): Heterodera AGROVOC | Nematoda | Yield losses | Ribosomal DNA | IranOnline resources: Access only for CIMMYT Staff In: Proceedings of the Sixth International Cereal Nematodes Symposium p. 45Summary: Cereal cyst nematodes (CCN), Heterodera avenae, H. latipons, and H. filipjevi, are considered major nematode species that cause significant yield losses of cereals around the world. Of these, H. filipjevi is widely spread in wheat fields in Iran, and causes yield losses. An in-silico study indicated that the sequences of the ITS regions of ribosomal DNA of H. filipjevi populations from Iran showed low similarity (97%) with sequences of the populations of the same species available in GenBank. Hence, further studies were conducted to clarify this phenomenon. DNA was extracted from 16 populations of H. filipjevi originating from six countries and included eight populations collected from different provinces in Iran. The ITS-rDNA region and mitochondrial cytochrome oxidase subunit 1 (COI) region were amplified and sequenced, and the obtained sequences were aligned. The alignment of the ITS region showed distinct differences in three positions between the Iranian and the other H. filipjevi populations (insertion of one nucleotide, 1 SNP, and a deletion of two nucleotides in another position). Also, the alignment of the COI sequences indicated clear differences between the Iranian populations and the other populations. Furthermore, deviating morphological characteristics for some of the Iranian H. filipjevi were noticeable, including variability in the underbridge (from “hardly observed” over “very weak” to “strongly developed”), and the bullae (from “very conspicuous and numerous” over “very weak and few” to “hardly found”). Further investigation is needed of the Iranian H. filipjevi populations to see to what extent these molecular and morphological variations reflect differences in pathogenicity and impact yield, and for discriminating the Iranian populations of H. filipjevi in different localities.
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Lic. Jose Juan Caballero Flores

 

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Cereal cyst nematodes (CCN), Heterodera avenae, H. latipons, and H. filipjevi, are considered major nematode species that cause significant yield losses of cereals around the world. Of these, H. filipjevi is widely spread in wheat fields in Iran, and causes yield losses. An in-silico study indicated that the sequences of the ITS regions of ribosomal DNA of H. filipjevi populations from Iran showed low similarity (97%) with sequences of the populations of the same species available in GenBank. Hence, further studies were conducted to clarify this phenomenon. DNA was extracted from 16 populations of H. filipjevi originating from six countries and included eight populations collected from different provinces in Iran. The ITS-rDNA region and mitochondrial cytochrome oxidase subunit 1 (COI) region were amplified and sequenced, and the obtained sequences were aligned. The alignment of the ITS region showed distinct differences in three positions between the Iranian and the other H. filipjevi populations (insertion of one nucleotide, 1 SNP, and a deletion of two nucleotides in another position). Also, the alignment of the COI sequences indicated clear differences between the Iranian populations and the other populations. Furthermore, deviating morphological characteristics for some of the Iranian H. filipjevi were noticeable, including variability in the underbridge (from “hardly observed” over “very weak” to “strongly developed”), and the bullae (from “very conspicuous and numerous” over “very weak and few” to “hardly found”). Further investigation is needed of the Iranian H. filipjevi populations to see to what extent these molecular and morphological variations reflect differences in pathogenicity and impact yield, and for discriminating the Iranian populations of H. filipjevi in different localities.

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