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A single copy of virus-derived, transgene-encoding hairpin RNA confers BYDY immunity

By: Abbott, D | Proceedimgs of an International Symposium Texcoco, Mex. (Mexico) 1-5 Sep 2002.
Contributor(s): Henry, M.|McNab, A [eds.] | Wang, M.B [coaut.] | Waterhouse, P [coaut.].
Material type: materialTypeLabelBookAnalytics: Show analyticsPublisher: Mexico, DF (Mexico) CIMMYT : 2002Description: p. 22-26.ISBN: 970-648-095-1.Subject(s): Barley yellow dwarf virus | Drosophila | Genetic resistance | Hordeum vulgare | Polymerase chain reaction | Quality | RNA | Seed production | transgenicsDDC classification: 632.3 Summary: The PAV serotype of Barley yellow dwarf virus (BYDV-PAV) is the most damaging virus of cereals worldwide. Natural resistance genes against the virus give inadequate control, and previous attempts to introduce synthetic resistance genes have been disappointing. The transformation of cereals with BYDV coat protein or polymerase genes has produced a few virus-resistant plants, but the resistance has been weak, unstable, or has failed to segregate as expected. In an attempt to generate barley with protection against BYDV; Golden Promise barley was transformed with a transgene that was designed to produce hairpin (hp)RNA from BYDV-PAV sequences. The majority of the resulting lines contained a single copy transgene insert and were rated as immune because the virus could not be detected in the challenged plants by ELISA nor recovered by aphid feeding experiments. In the field, BYDV-PAV is sometimes associated with the related luteovirus Cereal yellow dwarf virus (CYDV-RPV). When the transgenic plants were challenged with BYDV-PAV and CYDV together, the plants were susceptible to CYDV but immune to BYDV-PAV: This shows that the immunity is sequence-specific and not broken down by the presence of CYDV: It also suggests that the immunity will be robust in the field and very useful in minimizing losses in barley production due to BYDV-PAV.Collection: CIMMYT Publications Collection
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Book CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

CIMMYT Publications Collection 632.3 HEN (Browse shelf) 1 Available G629708
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The PAV serotype of Barley yellow dwarf virus (BYDV-PAV) is the most damaging virus of cereals worldwide. Natural resistance genes against the virus give inadequate control, and previous attempts to introduce synthetic resistance genes have been disappointing. The transformation of cereals with BYDV coat protein or polymerase genes has produced a few virus-resistant plants, but the resistance has been weak, unstable, or has failed to segregate as expected. In an attempt to generate barley with protection against BYDV; Golden Promise barley was transformed with a transgene that was designed to produce hairpin (hp)RNA from BYDV-PAV sequences. The majority of the resulting lines contained a single copy transgene insert and were rated as immune because the virus could not be detected in the challenged plants by ELISA nor recovered by aphid feeding experiments. In the field, BYDV-PAV is sometimes associated with the related luteovirus Cereal yellow dwarf virus (CYDV-RPV). When the transgenic plants were challenged with BYDV-PAV and CYDV together, the plants were susceptible to CYDV but immune to BYDV-PAV: This shows that the immunity is sequence-specific and not broken down by the presence of CYDV: It also suggests that the immunity will be robust in the field and very useful in minimizing losses in barley production due to BYDV-PAV.

English

0208|AGRIS 0401|AL-Wheat Program|R02CIMPU

Juan Carlos Mendieta

CIMMYT Publications Collection

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