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Advances in molecular markers for bread making quality

By: Dubcovsky, J | Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico DF (Mexico).
Contributor(s): Khan, I.A [coaut.] | Kohli, M.M.|Francis, M [eds.] | Lijavetzky, D [coaut.] | Manifesto, M.M [coaut.] | Marcucci-Poltri, S [coaut.] | Schlatter, A.R [coaut.] | Tranquilli, G [coaut.].
Material type: materialTypeLabelBookAnalytics: Show analyticsPublisher: Montevideo (Uruguay) CIMMYT : 2000Description: 57-70.ISBN: 9974-7586-1-0.Subject(s): Biotechnology | Food production | Genetic variation | Haploidy AGROVOC | South America | CIMMYT | Triticum | Plant breeding AGROVOCDDC classification: 633.1153 Summary: Composition of the grain storage proteins, grain protein content (GPC) and hardness are the major factors determining bread making quality. Though the effect of specific high molecular weight glutenin subunits on bread making performance is well characterized, they usually account for less than 50% of the inter-cultivar variation in gluten strength. A specific Gli-B 1/Glu-B3 allele from Klein 32 that accounts for a significant proportion of the variation in gluten strength has been recently identified using a microsatellite marker. Introgression of this allele in combination with appropriate high molecular weight glutenin subunits may allow a more efficient manipulation of gluten strength. Besides gluten strength, GPC is probably the major factor affecting bread making and pasta quality. In spite of the importance of this character; progress in breeding for high GPC has been slow and difficult because of the large effect of the environment and the strong negative relationship between grain protein percentage and grain yield. Molecular markers linked to a gene for high GPC have been recently identified in the short arm of Triticum dicoccoides chromosome 68. A large segment of this chromosome was detected in the high GPC hexaploid variety Glupro. Various molecular markers can be used to introgress the high GPC gene into both tetraploid and hexaploid wheat varieties. Manipulation of GPC and protein quality with molecular markers can be now complemented with the manipulation of grain hardness using similar techniques. Tight linkage between the grain softness related protein and the puroindoline-a loci (both associated with hardness) allow the combination of the molecular markers for both loci. A strategy combining PCR and restriction enzymes was developed to facilitate the introgression of different hardness alleles in marker assisted selection programs.Collection: CIMMYT Publications Collection
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Conference proceedings CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

CIMMYT Publications Collection 633.1153 KOH (Browse shelf) 1 Available B649448
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Composition of the grain storage proteins, grain protein content (GPC) and hardness are the major factors determining bread making quality. Though the effect of specific high molecular weight glutenin subunits on bread making performance is well characterized, they usually account for less than 50% of the inter-cultivar variation in gluten strength. A specific Gli-B 1/Glu-B3 allele from Klein 32 that accounts for a significant proportion of the variation in gluten strength has been recently identified using a microsatellite marker. Introgression of this allele in combination with appropriate high molecular weight glutenin subunits may allow a more efficient manipulation of gluten strength. Besides gluten strength, GPC is probably the major factor affecting bread making and pasta quality. In spite of the importance of this character; progress in breeding for high GPC has been slow and difficult because of the large effect of the environment and the strong negative relationship between grain protein percentage and grain yield. Molecular markers linked to a gene for high GPC have been recently identified in the short arm of Triticum dicoccoides chromosome 68. A large segment of this chromosome was detected in the high GPC hexaploid variety Glupro. Various molecular markers can be used to introgress the high GPC gene into both tetraploid and hexaploid wheat varieties. Manipulation of GPC and protein quality with molecular markers can be now complemented with the manipulation of grain hardness using similar techniques. Tight linkage between the grain softness related protein and the puroindoline-a loci (both associated with hardness) allow the combination of the molecular markers for both loci. A strategy combining PCR and restriction enzymes was developed to facilitate the introgression of different hardness alleles in marker assisted selection programs.

English

0105|AL-Wheat Program|AGRIS 0102

Jose Juan Caballero

CIMMYT Publications Collection

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Lunes –Viernes 9:00 am. 17:00 pm. Si tiene cualquier pregunta, contáctenos a CIMMYT-Knowledge-Center@cgiar.org