Knowledge Center Catalog

The dehydrin multigene family in triticeae and maize

By: Contributor(s): Material type: TextTextPublication details: Mexico, DF (Mexico) CIMMYT : 2000ISBN:
  • 970-648-052-8
Subject(s): DDC classification:
  • 631.53 RIB
Summary: One objective of our work has been to determine the map position of every member of the dehydrin (Dhn) gene family in barley and maize. The purpose is to provide a foundation for testing associations between alleles at specific Dhn loci and phenotypic variation related to drought and temperature responses. Dehydrins are a species of protein produced by plants during drought or low temperature stress. Eleven barley Dhn genes were identified, sequenced, and mapped ( using gene-specific PCR) to chromosomes 3H, 4H,5H, and 6H. Wheat Dhn genes were mapped to 4DS, 5BL, and 6AL using seed protein data from cytogenetic stocks. Six maize Dhn probes identified eight maize Dhn loci on chromosomes 1, 3, 4, 5, 6, and 9. Expression (determined by gene- specific RT -PCR) varied; some genes are induced by drought, others by low temperature. This variation, together with cross-hybridization between Dhn genes, highlights the necessity of gene-specific methods to study the Dhn multigene family.
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Item type Current library Collection Call number Copy number Status Date due Barcode Item holds
Conference proceedings CIMMYT Knowledge Center: John Woolston Library CIMMYT Publications Collection 631.53 RIB (Browse shelf(Opens below)) 1 Available X629165
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One objective of our work has been to determine the map position of every member of the dehydrin (Dhn) gene family in barley and maize. The purpose is to provide a foundation for testing associations between alleles at specific Dhn loci and phenotypic variation related to drought and temperature responses. Dehydrins are a species of protein produced by plants during drought or low temperature stress. Eleven barley Dhn genes were identified, sequenced, and mapped ( using gene-specific PCR) to chromosomes 3H, 4H,5H, and 6H. Wheat Dhn genes were mapped to 4DS, 5BL, and 6AL using seed protein data from cytogenetic stocks. Six maize Dhn probes identified eight maize Dhn loci on chromosomes 1, 3, 4, 5, 6, and 9. Expression (determined by gene- specific RT -PCR) varied; some genes are induced by drought, others by low temperature. This variation, together with cross-hybridization between Dhn genes, highlights the necessity of gene-specific methods to study the Dhn multigene family.

English

0101|AL-ABC Program|AL-Maize Program|AGRIS 0101

Jose Juan Caballero

CIMMYT Publications Collection


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