Normal view MARC view ISBD view

An analysis of the use of haploidy in wheat improvement

By: Mujeeb-Kazi, A | Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico DF (Mexico).
Contributor(s): Kohli, M.M.|Francis, M [eds.].
Material type: materialTypeLabelBookAnalytics: Show analyticsPublisher: Montevideo (Uruguay) CIMMYT : 2000Description: 33-48.Subject(s): Haploidy AGROVOC | Plant emasculation | CIMMYT | INIA | Triticum | Triticum aestivum AGROVOC | Wheat AGROVOC | Hybrids AGROVOC | Plant breeding AGROVOCSummary: Poly haploid production in wheat has relied heavily on anther culture and sexual crosses with Hordeum bulbosum. The occurrence of somaclonal variation, aneuploidy and genotypic specificity are major limitations of anther culture. The homoeologous group 5 crossability loci (Kr) influence the sexual crossings of wheat with H. bulbosum. Producing wheat haploids by sexual crosses of bread wheat x maize, pearl millet or Tripsacum has become a significant procedure since the production constraints of anther culture and H. bulbosum crosses are not present. Currently this procedure is being routinely used in wheat cytogenetics, wide crosses, wheat breeding, with extensions of the application into genetic engineering and molecular mapping. Recent technique advances in enhancing efficiency of haploid production utilize detached tillers from selected plants, their culture in a nutrient solution including sulfurous acid to avoid contamination, and hot water (43°C) immersion of the spikes for three minutes to effect emasculation. Hormonal treatment (2,4- dichlorophenoxy acetic acid,. 100 ppm) is essential, as is embryo rescue 14 days after pollination. The protocol is almost 100% effective for all bread wheat-cultivars. It has genotypic specificity for durums and TriticoSecale. The contribution of D genome chromosomes is a definitive factor in cross success. Mean frequencies of embryo excision are 25%, plantlet differentiation 80%, with a colchicine induced doubling range from 80 to 95%. Results of the techniques application in wide crosses, genetics, cytogenetics, breeding, and genetic transformation will be presented. Use of stored millet pollen to facilitate haploid production, due to unavailability of fresh pollen when wheat is grown, will be elucidated.Collection: CIMMYT Staff Publications Collection
Tags from this library: No tags from this library for this title. Log in to add tags.
    average rating: 0.0 (0 votes)
Item type Current location Collection Call number Copy number Status Date due Barcode Item holds
Conference proceedings CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

CIMMYT Staff Publications Collection CIS-2901 (Browse shelf) 1 Available 649450
Total holds: 0

Poly haploid production in wheat has relied heavily on anther culture and sexual crosses with Hordeum bulbosum. The occurrence of somaclonal variation, aneuploidy and genotypic specificity are major limitations of anther culture. The homoeologous group 5 crossability loci (Kr) influence the sexual crossings of wheat with H. bulbosum. Producing wheat haploids by sexual crosses of bread wheat x maize, pearl millet or Tripsacum has become a significant procedure since the production constraints of anther culture and H. bulbosum crosses are not present. Currently this procedure is being routinely used in wheat cytogenetics, wide crosses, wheat breeding, with extensions of the application into genetic engineering and molecular mapping. Recent technique advances in enhancing efficiency of haploid production utilize detached tillers from selected plants, their culture in a nutrient solution including sulfurous acid to avoid contamination, and hot water (43°C) immersion of the spikes for three minutes to effect emasculation. Hormonal treatment (2,4- dichlorophenoxy acetic acid,. 100 ppm) is essential, as is embryo rescue 14 days after pollination. The protocol is almost 100% effective for all bread wheat-cultivars. It has genotypic specificity for durums and TriticoSecale. The contribution of D genome chromosomes is a definitive factor in cross success. Mean frequencies of embryo excision are 25%, plantlet differentiation 80%, with a colchicine induced doubling range from 80 to 95%. Results of the techniques application in wide crosses, genetics, cytogenetics, breeding, and genetic transformation will be presented. Use of stored millet pollen to facilitate haploid production, due to unavailability of fresh pollen when wheat is grown, will be elucidated.

English

R99-00CIMPU|0011|EE|AL-Wheat Program|3

Jose Juan Caballero

CIMMYT Staff Publications Collection

There are no comments for this item.

Log in to your account to post a comment.
baner

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2015. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
Monday –Friday 9:00 am. 17:00 pm. If you have any question, please contact us at CIMMYT-Knowledge-Center@cgiar.org

Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT) © Copyright 2015. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
Lunes –Viernes 9:00 am. 17:00 pm. Si tiene cualquier pregunta, contáctenos a CIMMYT-Knowledge-Center@cgiar.org