Tagging Lr19 with AFLP markers
Material type:
TextPublication details: Addis Ababa (Ethiopia) CIMMYT : 1999ISBN: - 92-9146-058-3
| Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|---|
| Conference proceedings | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Publications Collection | Look under series title (Browse shelf(Opens below)) | 1 | Available | 1H629146 |
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| Look under series title The impact of crop rotation on the grain yield of rainfed wheat in Northern Zambia | Look under series title Sustainable dryland wheat production through green manuring | Look under series title The national cereal rust control program in Australia | Look under series title Tagging Lr19 with AFLP markers | Look under series title Detecting the Sr2-linked gene for seedling chlorosis in South African wheat cultivars | Look under series title Effect of heading date on stem rust development in durum wheat | Look under series title Evaluation of resistance of selected wheat cultivars to three isolates of Septoria tritici under field condition |
Despite reports of virulence in leaf rust (Puccinia recondita Rob. ex Desm. f. sp. tritici) to wheats carrying Lr19, this gene, which originates from Thinopyrum sp., remains an excellent source of resistance against all predominant South African pathotypes. A physical deletion map of the Lr 19 translocated chromosome facilitated the selection and characterisation of a white endosperm recombinant (LrI9-149). To achieve the aim of further shortening the Thinopyrum segment present in LrI9-149, and for subsequent selection and tagging of novel recombinants, genetic markers are required which lie closer to Lr 19 than any of the presently mapped marker loci. The AFLP technique has been employed to identify DNA sequences mapping in close proximity to Lr19, and these sequences can be used to design PCR based assays. Such assays are well suited to marker-assisted selection strategies.
English
0007|AGRIS 0101|AL-Wheat Program
Jose Juan Caballero
CIMMYT Publications Collection