Knowledge Center Catalog

Aggressiveness of Phaeosphaeria nodorum isolates and their in vitro secretion of cell-wall-degrading enzymes

By: Contributor(s): Material type: TextTextPublication details: Mexico, DF (Mexico) CIMMYT : 1999ISBN:
  • 970-648-035-8
Subject(s): DDC classification:
  • 632.4 GIN
Summary: The relationship between the in vitro production of cell-wall-degrading enzymes and the aggressiveness of three Phaeosphaeria nodorum isolates was studied. When grown in liquid medium containing 1% cell wall from wheat leaves as the only carbon source, the isolates secreted xylanase, ß-arabinosidase, ß-xylosidase, polygalacturonase, ß-galactosidase, cellulase, ß-1.3-glucanase, ß-glucosidase, acetyl-esterase, and butyrate-esterase. Time course experiments showed different levels of enzyme production and different kinetics between isolates. Xylanase, cellulase, polygalacturonase, and butyrate-esterase were positively correlated with isolate aggressiveness. The most aggressive isolate produced a higher proportion of xylanase than the other two isolates, suggesting the role of this enzyme in the pathogenesis of P. nodorum.
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Collection Call number Copy number Status Date due Barcode Item holds
Conference proceedings CIMMYT Knowledge Center: John Woolston Library CIMMYT Publications Collection 632.4 GIN (Browse shelf(Opens below)) 1 Available J628903
Total holds: 0

The relationship between the in vitro production of cell-wall-degrading enzymes and the aggressiveness of three Phaeosphaeria nodorum isolates was studied. When grown in liquid medium containing 1% cell wall from wheat leaves as the only carbon source, the isolates secreted xylanase, ß-arabinosidase, ß-xylosidase, polygalacturonase, ß-galactosidase, cellulase, ß-1.3-glucanase, ß-glucosidase, acetyl-esterase, and butyrate-esterase. Time course experiments showed different levels of enzyme production and different kinetics between isolates. Xylanase, cellulase, polygalacturonase, and butyrate-esterase were positively correlated with isolate aggressiveness. The most aggressive isolate produced a higher proportion of xylanase than the other two isolates, suggesting the role of this enzyme in the pathogenesis of P. nodorum.

English

9910|AGRIS 0001

Jose Juan Caballero

CIMMYT Publications Collection


International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org