Knowledge Center Catalog

Molecular analysis of a DNA fingerprint probe from Mycosphaerella graminicola

By: Contributor(s): Material type: TextTextPublication details: Mexico, DF (Mexico) CIMMYT : 1999ISBN:
  • 970-648-035-8
Subject(s): DDC classification:
  • 632.4 GIN
Summary: Clones hybridizing to the Mycosphaerella graminicola DNA fingerprint probe pSTL70 were identified from subgenomic libraries and sequenced. Analysis of the DNA sequences of these clones plus the original pSTL70 clone revealed that pSTL70 contains part of the open reading frame for a probable homologue of an osmosensing histidine kinase gene from yeast. The remaining portion of the clone contained a partial reverse transcriptase gene sequence and a 29 base pair direct repeat, which could mean that the clone is a transposable element. Methods for converting transposable elements into improved DNA fingerprinting techniques are discussed.
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Item type Current library Collection Call number Copy number Status Date due Barcode Item holds
Conference proceedings CIMMYT Knowledge Center: John Woolston Library CIMMYT Publications Collection 632.4 GIN (Browse shelf(Opens below)) 1 Available C628903
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Clones hybridizing to the Mycosphaerella graminicola DNA fingerprint probe pSTL70 were identified from subgenomic libraries and sequenced. Analysis of the DNA sequences of these clones plus the original pSTL70 clone revealed that pSTL70 contains part of the open reading frame for a probable homologue of an osmosensing histidine kinase gene from yeast. The remaining portion of the clone contained a partial reverse transcriptase gene sequence and a 29 base pair direct repeat, which could mean that the clone is a transposable element. Methods for converting transposable elements into improved DNA fingerprinting techniques are discussed.

English

9910|AGRIS 0001

Jose Juan Caballero

CIMMYT Publications Collection


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