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Characterization of Cochliobolus sativus isolates from the UK and Yemen

By: Contributor(s): Material type: TextTextPublication details: Mexico, DF (Mexico) CIMMYT|UCL|BADC : 1998ISBN:
  • 970-648-001-3
Subject(s): DDC classification:
  • 633.1194 DUV
Summary: The properties of six isolates of Cochliobolus sativus (Bipolaris sorokiniana) from barley in Scotland, one isolate from Yemeni barley with black point disease, and one from Yemeni wheat with black point disease were compared. The eight isolates differ in pathogenicity, conidia size, growth rate, and response to temperature; only the isolate from Yemeni wheat was able to grow well at 35ºC. All isolates were all able to produce xylanase when grown in culture. To compare genes coding for cell-wall-degrading enzymes in the different isolates, total DNA was digested with several restriction enzymes and probed with XYL1 and PGN1 cDNAs of C. carbon. Southern hybridization patterns were similar for all isolates except the one from Yemeni wheat, and were distinct from those obtained with DNA of the related species C. heterostrophus. DNA of all eight isolates and of C. heterostrophus could be amplified by the polymerase chain reaction (PCR) with sequences from the C. sativus XYL2 gene as rimers. DNA of most isolates gave two bands; sequence analysis showed that these correspond to the XYL2 gene (with one intron) and the XYL2 gene (with two introns) of C. carbonum. DNA of the isolate from Yemeni wheat, however, also gave a third, smaller band . DNA of C. heterostrophus gave two bands, but one corresponds to XYL1 and the other co-migrates with the third band of the Yemeni wheat isolate of C. sativus.
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Conference proceedings CIMMYT Knowledge Center: John Woolston Library CIMMYT Publications Collection 633.1194 DUV (Browse shelf(Opens below)) 1 Available 1U624337
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The properties of six isolates of Cochliobolus sativus (Bipolaris sorokiniana) from barley in Scotland, one isolate from Yemeni barley with black point disease, and one from Yemeni wheat with black point disease were compared. The eight isolates differ in pathogenicity, conidia size, growth rate, and response to temperature; only the isolate from Yemeni wheat was able to grow well at 35ºC. All isolates were all able to produce xylanase when grown in culture. To compare genes coding for cell-wall-degrading enzymes in the different isolates, total DNA was digested with several restriction enzymes and probed with XYL1 and PGN1 cDNAs of C. carbon. Southern hybridization patterns were similar for all isolates except the one from Yemeni wheat, and were distinct from those obtained with DNA of the related species C. heterostrophus. DNA of all eight isolates and of C. heterostrophus could be amplified by the polymerase chain reaction (PCR) with sequences from the C. sativus XYL2 gene as rimers. DNA of most isolates gave two bands; sequence analysis showed that these correspond to the XYL2 gene (with one intron) and the XYL2 gene (with two introns) of C. carbonum. DNA of the isolate from Yemeni wheat, however, also gave a third, smaller band . DNA of C. heterostrophus gave two bands, but one corresponds to XYL1 and the other co-migrates with the third band of the Yemeni wheat isolate of C. sativus.

English

9806|AGRIS 9802

Jose Juan Caballero

CIMMYT Publications Collection


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