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Biochemical characterization of drought tolerant and susceptible young maize plants under well-watered conditions

By: Pena-Valdivia, C.B | Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico DF (Mexico).
Contributor(s): Edmeades, G.O.|Banziger, M.|Mickelson, H.R.|Pena-Valdivia, C.B [eds.] | Lotina H., B [coaut.] | Merchant, R [coaut.] | Perales Rivera, H [coaut.] | Raya P., J.C [coaut.].
Material type: materialTypeLabelBookAnalytics: Show analyticsPublisher: Mexico, DF (Mexico) CIMMYT : 1997ISBN: 968-6923-93-4.Subject(s): Biochemistry | Drought resistance | Drought stress AGROVOC | Photosynthesis AGROVOC | Resistance to injurious factors | Selection | CIMMYT | Zea mays AGROVOCDDC classification: 633.153 Summary: Selected biochemical traits were evaluated in various sections of developing leaves of a drought susceptible population, Tuxpeño Sequia C0, and of a drought-tolerant selection from the same population, Tuxpeño Sequia C8. Both populations were grown in the greenhouse under full sunlight and well-watered conditions. Photosynthetic and transpiration rates, a and b chlorophyll content, total protein, dry matter and specific weight were measured in five 2 cm sections along the second leaf when it was 10 cm long. Chloroplasts from mesophyll cells were also isolated from each section and photochemical activities were measured. Finally, ultrastructure organization of the chloroplasts in leaf tissue was observed by electron microscopy. Tuxpeño Sequia C8 had a higher photosynthetic rate (30%), accumulated more dry matter per leaf segment (5%), and had a higher specific leaf weight (10.5%) than C0. Although total protein and chlorophyll a contents were similar in both populations, C8 had 19% less chlorophyll b. With respect to photochemical activities of isolated chloroplasts, values for proton pumping, photophosphorylation and basal phosphorylating and uncoupled electron transport rates were 60 to 80% lower in C8 than in C0. Electron microscopy showed that chloroplasts of both blundle sheath and mesophyll cells differed somewhat morphologically between the populations.Collection: CIMMYT Publications Collection
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Conference proceedings CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

CIMMYT Publications Collection 633.153 EDM (Browse shelf) 1 Available 1G624179
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Selected biochemical traits were evaluated in various sections of developing leaves of a drought susceptible population, Tuxpeño Sequia C0, and of a drought-tolerant selection from the same population, Tuxpeño Sequia C8. Both populations were grown in the greenhouse under full sunlight and well-watered conditions. Photosynthetic and transpiration rates, a and b chlorophyll content, total protein, dry matter and specific weight were measured in five 2 cm sections along the second leaf when it was 10 cm long. Chloroplasts from mesophyll cells were also isolated from each section and photochemical activities were measured. Finally, ultrastructure organization of the chloroplasts in leaf tissue was observed by electron microscopy. Tuxpeño Sequia C8 had a higher photosynthetic rate (30%), accumulated more dry matter per leaf segment (5%), and had a higher specific leaf weight (10.5%) than C0. Although total protein and chlorophyll a contents were similar in both populations, C8 had 19% less chlorophyll b. With respect to photochemical activities of isolated chloroplasts, values for proton pumping, photophosphorylation and basal phosphorylating and uncoupled electron transport rates were 60 to 80% lower in C8 than in C0. Electron microscopy showed that chloroplasts of both blundle sheath and mesophyll cells differed somewhat morphologically between the populations.

English

9801|AGRIS 9702

Jose Juan Caballero

CIMMYT Publications Collection

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