Knowledge Center Catalog

Construction of a bioinsecticidal strain of Pseudomonas fluorescens active against sugarcane borer

By: Contributor(s): Material type: TextTextPublication details: Mexico, DF (Mexico) CIMMYT : 1997ISBN:
  • 968-6923-79-9
Subject(s): DDC classification:
  • 633.153 MIH
Summary: A crylA(c) gene was cloned from a native Bacillus thuringiensis strain which showed activity against the sugarcane borer Eldana saccharina. The sequence of the cloned gene was very similar to that of the B. thuringiensis subsp. kurstaki HD-73 crylA(c) gene. The gene was introduced into an isolate of Pseudomonas fluorescens capable of colonizing sugarcane, 071 two broad host range plasmids, pDER405 and pKT240, having copy numbers of 13 and 28 respectively. The cry gene was introduced into the chromosome of P. fluorescens isolate 14 Using an artificial transposon carrying vector, Omegon-Km. Bioassays on Eldana larvae showed that the strain carrying the gene integrated into the chromosome was as toxic as the one carrying it on pKT240. Glasshouse trials indicated that sugarcane treated with P. fluorescens 14::0megonl-Km-cry were more resistant to Eldana damage than untreated sugarcane.
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A crylA(c) gene was cloned from a native Bacillus thuringiensis strain which showed activity against the sugarcane borer Eldana saccharina. The sequence of the cloned gene was very similar to that of the B. thuringiensis subsp. kurstaki HD-73 crylA(c) gene. The gene was introduced into an isolate of Pseudomonas fluorescens capable of colonizing sugarcane, 071 two broad host range plasmids, pDER405 and pKT240, having copy numbers of 13 and 28 respectively. The cry gene was introduced into the chromosome of P. fluorescens isolate 14 Using an artificial transposon carrying vector, Omegon-Km. Bioassays on Eldana larvae showed that the strain carrying the gene integrated into the chromosome was as toxic as the one carrying it on pKT240. Glasshouse trials indicated that sugarcane treated with P. fluorescens 14::0megonl-Km-cry were more resistant to Eldana damage than untreated sugarcane.

English

9711|AGRIS 9702

Jose Juan Caballero

CIMMYT Publications Collection


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