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Mechanisms and bases of resistance in maize to southwestern corn borer and fall armyworm

By: Williams, W.P | Centro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico DF (Mexico).
Contributor(s): Davis, F.M [coaut.] | Mihm, J.A [ed.].
Material type: materialTypeLabelBookAnalytics: Show analyticsPublisher: Mexico, DF (Mexico) CIMMYT : 1997ISBN: 968-6923-79-9.Subject(s): Germplasm | Pest insects | Pest resistance | Spodoptera frugiperda | Tissue culture | CIMMYT | Zea mays AGROVOC | Genotypes AGROVOCDDC classification: 633.153 Summary: Maize, Zea mays L., germplasm lines with resistance to leaf feeding by the southwestern corn borer (SWCB), Diatraea grandiosella Dyar, and fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), have been developed and released. A series of experiments were conducted to determine the mechanisms and bases of this resistance. Field experiments have shown that antibiosis is a mechanism of resistance to both insects. When resistant and susceptible maize genotypes were infested with either SWCB or FAW neonates, the larvae that were recovered 10 to 14 days later from susceptible genotypes weighed twice as much as those recovered from resistant genotypes. Laboratory experiments using excised leaf tissue and liquid pressed from leaves demonstrated that larval non-preference is also a mechanism of resistance of these germplasm lines. When experiments were conducted using callus tissue of susceptible and resistant maize genotypes, both SWCB and FAW larvae preferred to feed on callus of susceptible genotypes. Larvae that fed on the susceptible calli weighed twice as much as those that fed on resistant calli. Similar differences in size were observed when larvae were fed on Lyophilized leaf tissue of susceptible and resistant genotypes. Factors responsible for these differences in growth are not fully known; however, leaf tissue from the inner whorls of resistant genotypes tends to be tougher than that from susceptible genotypes. The cuticle and epidermal cell wall of resistant genotypes is generally thicker. Leaves of resistant plants have a higher fiber content and lower total protein content. A 33kD polypeptide found in callus tissue appears to be associated with resistant genotypes. Electrophoretic analysis of whorl leaf tissue also indicated a possible association of resistance to SWCB and FAW with 21kD and 36kD polypeptides.Collection: CIMMYT Publications Collection
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Conference proceedings CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

CIMMYT Publications Collection 633.153 MIH (Browse shelf) 1 Available D623915
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Maize, Zea mays L., germplasm lines with resistance to leaf feeding by the southwestern corn borer (SWCB), Diatraea grandiosella Dyar, and fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), have been developed and released. A series of experiments were conducted to determine the mechanisms and bases of this resistance. Field experiments have shown that antibiosis is a mechanism of resistance to both insects. When resistant and susceptible maize genotypes were infested with either SWCB or FAW neonates, the larvae that were recovered 10 to 14 days later from susceptible genotypes weighed twice as much as those recovered from resistant genotypes. Laboratory experiments using excised leaf tissue and liquid pressed from leaves demonstrated that larval non-preference is also a mechanism of resistance of these germplasm lines. When experiments were conducted using callus tissue of susceptible and resistant maize genotypes, both SWCB and FAW larvae preferred to feed on callus of susceptible genotypes. Larvae that fed on the susceptible calli weighed twice as much as those that fed on resistant calli. Similar differences in size were observed when larvae were fed on Lyophilized leaf tissue of susceptible and resistant genotypes. Factors responsible for these differences in growth are not fully known; however, leaf tissue from the inner whorls of resistant genotypes tends to be tougher than that from susceptible genotypes. The cuticle and epidermal cell wall of resistant genotypes is generally thicker. Leaves of resistant plants have a higher fiber content and lower total protein content. A 33kD polypeptide found in callus tissue appears to be associated with resistant genotypes. Electrophoretic analysis of whorl leaf tissue also indicated a possible association of resistance to SWCB and FAW with 21kD and 36kD polypeptides.

English

9711|AGRIS 9702

Jose Juan Caballero

CIMMYT Publications Collection

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Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT) © Copyright 2015. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
Lunes –Viernes 9:00 am. 17:00 pm. Si tiene cualquier pregunta, contáctenos a CIMMYT-Knowledge-Center@cgiar.org