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High genetic diversity of Mycospaherella graminicola (Zymoseptoria tritici) from a single wheat field in Tunisia as revealed by SSR markers

By: Contributor(s): Material type: ArticleArticlePublication details: 2013ISSN:
  • 1684-5315
Subject(s): Online resources: In: African Journal of Biotechnology v. 12, no. 12, p. 1344-1349Summary: Microsatellite markers were used to assess genetic diversity of Mycosphaerella graminicola at a micro geographical scale in Tunisia. Forty five (45) isolates were sampled and assessed using seven pairs of single-locus microsatellite primers not previously tested on populations of M. graminicola in Tunisia. Genetic diversity of the field population ranged from 0.403 to 0.555 with an average of 0.484. A high level of genetic diversity was found at a sharp scale throughout the pathogen population tested. Among 45 isolates sampled, 39 different multi locus genotypes (MLG) were identified. Cluster analysis (UPGMA) showed that 86% of the isolates tested were distinct. The high degree of DNA polymorphism, the large number of different molecular genotypes and the pattern of cluster analysis suggest that sexual ascospores and/or asexual spores of a highly mutable local population could have contributed to the genetic diversity of M. graminicola in Tunisia.
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Item type Current library Collection Call number Status Date due Barcode Item holds
Article CIMMYT Knowledge Center: John Woolston Library CIMMYT Staff Publications Collection CIS-7204 (Browse shelf(Opens below)) Available
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Peer-review: No - Open Access: Yes|http://www.ajol.info/index.php/ajb

Peer review

Open Access

Microsatellite markers were used to assess genetic diversity of Mycosphaerella graminicola at a micro geographical scale in Tunisia. Forty five (45) isolates were sampled and assessed using seven pairs of single-locus microsatellite primers not previously tested on populations of M. graminicola in Tunisia. Genetic diversity of the field population ranged from 0.403 to 0.555 with an average of 0.484. A high level of genetic diversity was found at a sharp scale throughout the pathogen population tested. Among 45 isolates sampled, 39 different multi locus genotypes (MLG) were identified. Cluster analysis (UPGMA) showed that 86% of the isolates tested were distinct. The high degree of DNA polymorphism, the large number of different molecular genotypes and the pattern of cluster analysis suggest that sexual ascospores and/or asexual spores of a highly mutable local population could have contributed to the genetic diversity of M. graminicola in Tunisia.

Global Wheat Program

English

CIMMYT Informa No. 1853| Academic Journals

INT3301

CIMMYT Staff Publications Collection

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