Normal view MARC view ISBD view

Identification and pathogenicity assessment of Fusarium spp. sampled from durum wheat fields in Tunisia

By: Fakhfakh, M.M.
Contributor(s): Daaloul, A [coaut.] | Elias, E.M [coaut.] | Rezgui, S [coaut.] | Yahyaoui, A.H [coaut.].
Material type: materialTypeLabelArticlePublisher: 2011ISSN: No (Revista en electrónico); 1684?5315.Subject(s): Durum wheat | Fusarium head blight (FHB) | Fusarium species | Pathogenicity In: African Journal of Biotechnology v. 10, no. 34, p. 6529-6539Summary: Durum wheat is the major cereal crop cultivated in Tunisia; covering over 40% of the cereal growing areas. Durum wheat production remains below expectation due to its low productivity that is attributed to the chronically abiotic and biotic stresses. Fusarium head blight (FHB) caused by Fusarium spp. Has become an important disease on durum wheat (Triticum turgidum L var. durum) in Tunisia, particularly during humid season. To identity the Fusarium species associated with FHB, samples were collected from five and six durum wheat fields in Northern Tunisia during 2004 and 2007 crop seasons, respectively. A total of 241 single spore cultures were isolated and seven different Fusarium spp. Were identified using morphological traits and species-specific PCR assays. Pathogenicity of FHB causal agents was assessed on predominant durum wheat varieties. Aggressiveness of selected Fusarium isolates was investigated. Three durum wheat cultivars, characterized by different level of susceptibility to FHB, were artificially inoculated. Symptoms of FHB were rated as percentage of infected spikelets (PIS) at 7, 14 and 21 days after inoculation (DAI) and area under disease progress curve (AUDPC) was analysed. Fusarium culmorum was the dominant species representing 36.3% of all sampled isolates. All Fusarium species tested caused visible infections to the durum wheat cultivars with significant difference in aggressiveness among species. F. culmorum was considered highly pathogenic with an AUDPC=243.7, followed by Fusarium pseudograminearum (AUDPC=216.7). The remaining species, which had <150 AUDPC, were moderately and weakly pathogenic. There were significant differences (P < 0.05) in aggressiveness among isolates within species suggesting that screening for resistance to FHB requires a mixture of several isolates within and among Fusarium species.Collection: Reprints Collection
Tags from this library: No tags from this library for this title. Log in to add tags.
    average rating: 0.0 (0 votes)
Item type Current location Collection Call number Status Date due Barcode Item holds
Article CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

Reprints Collection Available
Total holds: 0

Peer-review: No - Open Access: Yes|http://www.ajol.info/index.php/ajb

Durum wheat is the major cereal crop cultivated in Tunisia; covering over 40% of the cereal growing areas. Durum wheat production remains below expectation due to its low productivity that is attributed to the chronically abiotic and biotic stresses. Fusarium head blight (FHB) caused by Fusarium spp. Has become an important disease on durum wheat (Triticum turgidum L var. durum) in Tunisia, particularly during humid season. To identity the Fusarium species associated with FHB, samples were collected from five and six durum wheat fields in Northern Tunisia during 2004 and 2007 crop seasons, respectively. A total of 241 single spore cultures were isolated and seven different Fusarium spp. Were identified using morphological traits and species-specific PCR assays. Pathogenicity of FHB causal agents was assessed on predominant durum wheat varieties. Aggressiveness of selected Fusarium isolates was investigated. Three durum wheat cultivars, characterized by different level of susceptibility to FHB, were artificially inoculated. Symptoms of FHB were rated as percentage of infected spikelets (PIS) at 7, 14 and 21 days after inoculation (DAI) and area under disease progress curve (AUDPC) was analysed. Fusarium culmorum was the dominant species representing 36.3% of all sampled isolates. All Fusarium species tested caused visible infections to the durum wheat cultivars with significant difference in aggressiveness among species. F. culmorum was considered highly pathogenic with an AUDPC=243.7, followed by Fusarium pseudograminearum (AUDPC=216.7). The remaining species, which had <150 AUDPC, were moderately and weakly pathogenic. There were significant differences (P < 0.05) in aggressiveness among isolates within species suggesting that screening for resistance to FHB requires a mixture of several isolates within and among Fusarium species.

Global Wheat Program

English

WBR 2011|No CIMMYT affiliation| Academic Journals

Lucia Segura

INT3301

Reprints Collection

There are no comments for this item.

Log in to your account to post a comment.
baner

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2015. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
Monday –Friday 9:00 am. 17:00 pm. If you have any question, please contact us at CIMMYT-Knowledge-Center@cgiar.org

Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT) © Copyright 2015. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
Lunes –Viernes 9:00 am. 17:00 pm. Si tiene cualquier pregunta, contáctenos a CIMMYT-Knowledge-Center@cgiar.org