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Molecular characterization of genes associated with gluten strength, yellow pigment content and PPO activity in Chinese wheat cultivars

By: Contributor(s): Material type: ArticleArticleLanguage: Chinese Publication details: China : CNKI, 2008.ISSN:
  • 1009-1041
Subject(s): Online resources: In: Journal of Triticeae Crops v. 28, no. 2, p. 220-226635378Summary: Characterization of wheat quality genes is of great importance in quality improvement and molecular breeding. In the present study, 5 gene specific markers for HMW-GSDx5 and By8, 1B/1R translocation, Psy-A1 and Ppo-A1 genes were used to detect the allelic variations at these loci in 157 Chinese wheat cultivars and advanced lines. The results indicated that different frequencies were detected for the allelic variations of Dx5 and By8, 1B/1R translocations, Psy-A1 and Ppo-A1 genes in the wheat cultivars and advanced lines tested. The number of cultivars was 29, 68, 69, 42 and 78 with Dx5, By8, 1B/1R, Psy-A1b related to low yellow pigment content and Ppo-A1 associated with low PPO activity, and the frequencies accounted for 18.5%, 43.3% , 43.9%, 26.8% and 49.7%, respectively. Cultivars with all desired alleles for the five genes were presented only in one genotype. It is vital to combine several quality genes into a cultivar. The markers employed in this study were all gene-specific, with good repeatability and accuracy, and thus could be used as efficient molecular tools for marker-assisted selection (MAS) in wheat quality improvement programs.
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Characterization of wheat quality genes is of great importance in quality improvement and molecular breeding. In the present study, 5 gene specific markers for HMW-GSDx5 and By8, 1B/1R translocation, Psy-A1 and Ppo-A1 genes were used to detect the allelic variations at these loci in 157 Chinese wheat cultivars and advanced lines. The results indicated that different frequencies were detected for the allelic variations of Dx5 and By8, 1B/1R translocations, Psy-A1 and Ppo-A1 genes in the wheat cultivars and advanced lines tested. The number of cultivars was 29, 68, 69, 42 and 78 with Dx5, By8, 1B/1R, Psy-A1b related to low yellow pigment content and Ppo-A1 associated with low PPO activity, and the frequencies accounted for 18.5%, 43.3% , 43.9%, 26.8% and 49.7%, respectively. Cultivars with all desired alleles for the five genes were presented only in one genotype. It is vital to combine several quality genes into a cultivar. The markers employed in this study were all gene-specific, with good repeatability and accuracy, and thus could be used as efficient molecular tools for marker-assisted selection (MAS) in wheat quality improvement programs.

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