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PCR analysis of the cryI insecticidal crystal family genes from Bacillus thuringiensis

By: Ceron, J.
Contributor(s): Aranda, E [coaut.] | Bravo, A [coaut.] | Covarrubias, L [coaut.] | Lina, L [coaut.] | Ortiz, A [coaut.] | Ortiz, M [coaut.] | Quintero, R [coaut.].
Material type: materialTypeLabelArticlePublisher: 1994ISSN: 1098-5336 (Revista en electrónico); 0099-2240.Subject(s): Bacillaceae | Bacillus | Bacteria | Biological control organisms | Biopesticides | Cell structure | Chromosomes | Microbial pesticides | Nucleus | Pesticides | Pests of animals | Pests of plants | Toxic substances | ToxinsDDC classification: 95-088728 In: Applied and Environmental Microbiology v. 60, no. 1, p. 353-356Summary: A method allowing rapid and accurate identification of different subgroups within the insecticidal crystal CryI protein-producing family of Bacillus thuringiensis strains was established by using PCR technology. Thirteen highly homologous primers specific to regions within genes encoding seven different subgroups of B. thuringiensis CryI proteins were described. Differentiation among these strains was determined on the basis of the electrophoretic patterns of PCR products. B. thuringiensis strains, isolated from soil samples, were analyzed by PCR technology. Small amounts of bacterial lysates were assayed in two reaction mixtures containing six to eight primers. This method can be applied to rapidly detect the subgroups of CryI proteins that correspond with toxicity to various lepidopteran insectsCollection: AGRIS Collection
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Article CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

AGRIS Collection 95-088728 (Browse shelf) Available
Total holds: 0

Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0099-2240

references US (DNAL 448.3 Ap5)

A method allowing rapid and accurate identification of different subgroups within the insecticidal crystal CryI protein-producing family of Bacillus thuringiensis strains was established by using PCR technology. Thirteen highly homologous primers specific to regions within genes encoding seven different subgroups of B. thuringiensis CryI proteins were described. Differentiation among these strains was determined on the basis of the electrophoretic patterns of PCR products. B. thuringiensis strains, isolated from soil samples, were analyzed by PCR technology. Small amounts of bacterial lysates were assayed in two reaction mixtures containing six to eight primers. This method can be applied to rapidly detect the subgroups of CryI proteins that correspond with toxicity to various lepidopteran insects

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AGRIS Collection

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