An improved inoculation technique for sheath blight of rice caused by Rhizoctonia solani
Material type:
ArticleLanguage: English Publication details: United Kingdom : Taylor and Francis, 2002.ISSN: - 1715-2992 (Online)
- 0706-0661
| Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|---|
| Article | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Staff Publications Collection | CIS-4063 (Browse shelf(Opens below)) | 1 | Available | 630005 |
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Peer review
Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0706-0661
To develop a simple and reliable inoculation technique allowing to differentiate minor differences in susceptibility of rice cultivars to sheath blight caused by Rhizoctonia solani Kühn, the effects of different pathogen and host factors on disease development were investigated under greenhouse conditions. The most critical factor affecting uniform development of sheath blight was the amount of inoculum. Approximately 0.2 mg of inoculum placed inside the leaf sheath with a few drops of sterile water invariably induced single discrete, uniform-sized lesions, irrespective of the type of inoculum used (i.e., mature sclerotium, immature sclerotium, or mycelium). Considering the ease of maintaining uniformity of inoculum, the use of immature sclerotia is recommended. This inoculation method is not only simple, rapid, and highly reproducible, but it also allows to distinguish minor differences in susceptibility under greenhouse conditions.
Research and Partnership Program
Text in English
0406|AL-Wheat Program
INT1237
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