Normal view MARC view ISBD view

Development and use of cDNA probes in studies of barley yellow dwarf virus

By: Lister, R.M | World Perspectives on Barley Yellow Dwarf International Workshop Udine (Italy) 6-11 Jul 1987.
Contributor(s): Barbara, D.J [coaut.] | Fattouh, F [coaut.] | Kawata, E.E [coaut.] | Larkins, B.A [coaut.] | Ueng, P.P [coaut.].
Material type: materialTypeLabelBookAnalytics: Show analyticsPublisher: 1990ISBN: 968-6127-39.Subject(s): Acids | Aphididae | Aphidoidea | Arthropoda | Breeding methods | Carbohydrates | Cell structure | Cytoplasm | Cytoplasmic organelles | Glycosides | Hemiptera | Homoptera | Immunoenzyme techniques | Immunological techniques | Insecta | Luteoviruses | Nucleic acids | Nucleic compounds | Organic acids | Plant diseases | Plant viruses | Progeny | Protoplasm | Pure lines | Sternorrhyncha | Viruses AGROVOCDDC classification: 91-013767 In: Burnett, P.A. (ed.). World perspectives on barley yellow dwarf. Mexico, DF (Mexico). CIMMYT. 1990. p. 186-19391-013767Summary: Libraries of cDNA clones were produced from the RNAs of the MAV, RPV, and Purdue (P)-PAV isolates of barley yellow dwarf virus (BYDV) in bacteriophage gtll, by random priming. Screening with antisera to the isolates allowed selection of the recombinants capable of expressing capsid protein. With MAV, subcloning into the plasmid pUC18, followed by restriction endonuclease mapping, identified overlapping inserts collectively representing at least 85 % (a total of 5.1 kbp) of the BYDV genome. Immunologically positive clones shared a common region of approximately 1000 bp, located between 750 bp and 1750 bp from the 3' terminus of the genome. NucleotideCollection: AGRIS Collection
Tags from this library: No tags from this library for this title. Log in to add tags.
    average rating: 0.0 (0 votes)
Item type Current location Collection Call number Copy number Status Date due Barcode Item holds
Reprint Reprint CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

AGRIS Collection 91-013767 (Browse shelf) 1 Available 91-013767
Total holds: 0

14 ref

Libraries of cDNA clones were produced from the RNAs of the MAV, RPV, and Purdue (P)-PAV isolates of barley yellow dwarf virus (BYDV) in bacteriophage gtll, by random priming. Screening with antisera to the isolates allowed selection of the recombinants capable of expressing capsid protein. With MAV, subcloning into the plasmid pUC18, followed by restriction endonuclease mapping, identified overlapping inserts collectively representing at least 85 % (a total of 5.1 kbp) of the BYDV genome. Immunologically positive clones shared a common region of approximately 1000 bp, located between 750 bp and 1750 bp from the 3' terminus of the genome. Nucleotide

English

World Perspectives on Barley Yellow Dwarf International Workshop. Udine (Italy). 6-11 Jul 1987 CIMMYT, Ap. 6-641, 06600 Mexico, D.F. - Mexico|COMOD

AGRIS Collection

There are no comments for this item.

Log in to your account to post a comment.
baner

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2015. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
Monday –Friday 9:00 am. 17:00 pm. If you have any question, please contact us at CIMMYT-Knowledge-Center@cgiar.org

Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT) © Copyright 2015. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
Lunes –Viernes 9:00 am. 17:00 pm. Si tiene cualquier pregunta, contáctenos a CIMMYT-Knowledge-Center@cgiar.org