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Differentiation of barley yellow dwarf virus with monoclonal antibodies by immunosorbent electron microscopy

By: Contributor(s): Material type: TextTextLanguage: En Publication details: 1990ISBN:
  • 968-6127-39
Subject(s): DDC classification:
  • 91-013768
 In: Burnett, P.A. (ed.). World perspectives on barley yellow dwarf. Mexico, DF (Mexico). CIMMYT. 1990. p. 194-19691-013768Summary: Three strain-specific monoclonal antibodies (McAb), MAC 91, MAC 92, and MAFF2, were compared with two polyclonal antisera for the diagnosis of BYDV strains in immunosorbent electron microscopy. Used at an optimal dilution (1 ug/ml), the three McAbs specifically trapped the Rothamsted PAV-, RPV- and MAV-like isolates, unlike the polyclonal antisera, which showed considerable heterologous reaction. The test provides a simple and sensitive diagnostic method for BYDV, suitable for small immunosorbent assay results
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Three strain-specific monoclonal antibodies (McAb), MAC 91, MAC 92, and MAFF2, were compared with two polyclonal antisera for the diagnosis of BYDV strains in immunosorbent electron microscopy. Used at an optimal dilution (1 ug/ml), the three McAbs specifically trapped the Rothamsted PAV-, RPV- and MAV-like isolates, unlike the polyclonal antisera, which showed considerable heterologous reaction. The test provides a simple and sensitive diagnostic method for BYDV, suitable for small immunosorbent assay results

English

World Perspectives on Barley Yellow Dwarf International Workshop. Udine (Italy). 6-11 Jul 1987 CIMMYT, Ap. 6-641, 06600 Mexico, D.F. - Mexico|COMOD

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