Knowledge Center Catalog

Virus-like ["Cryptic"] particles present in healthy cereal tissue: Partial characterization and preparation of specific monoclonal antibody

By: Contributor(s): Material type: TextTextLanguage: En Publication details: 1990ISBN:
  • 968-6127-39
Subject(s): DDC classification:
  • 91-013875
In: Burnett, P.A. (ed.). World perspectives on barley yellow dwarf. Proceedings. Mexico, DF (Mexico). CIMMYT. 1990. p. 139-14391-013875Summary: Virus-like particles were isolated in low yields (1 to 20 ug/kg) from leaf and root tissue of a number of lines of barley (Hordeum vulgare L.), oats (Avena sativa L.), and wheat (Triticum aestivum L.) grown under conditions that excluded the introduction of known viruses by seed, soil, water contact, or insects. Extracted particles separated by rate-zonal centrifugation into discrete peaks were spherical (13.5 nm/50 S; 18 nm/72 S; 25 nm/118 S), germinate (24 x 14 nm/72 S), and "triplet" (36 x 14 nm/90 S). Germinate and triplet particles banded in gradients of cesium sulfate at 1.29 g/cm-3; a band of 10 % -shrunken particles formed at 1.31 g/cm-3.
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Virus-like particles were isolated in low yields (1 to 20 ug/kg) from leaf and root tissue of a number of lines of barley (Hordeum vulgare L.), oats (Avena sativa L.), and wheat (Triticum aestivum L.) grown under conditions that excluded the introduction of known viruses by seed, soil, water contact, or insects. Extracted particles separated by rate-zonal centrifugation into discrete peaks were spherical (13.5 nm/50 S; 18 nm/72 S; 25 nm/118 S), germinate (24 x 14 nm/72 S), and "triplet" (36 x 14 nm/90 S). Germinate and triplet particles banded in gradients of cesium sulfate at 1.29 g/cm-3; a band of 10 % -shrunken particles formed at 1.31 g/cm-3.

English

World Perspectives on Barley Yellow Dwarf International Workshop. Udine (Italy). 6-11 Jul 1987 CIMMYT, Ap. 6-641, 06600 Mexico, D.F. - Mexico|COMOD

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