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Identification, characterization, and utilization of sources of resistance to barley yellow dwarf virus

By: Contributor(s): Material type: TextTextLanguage: En Publication details: 1990ISBN:
  • 968-6127-39
Subject(s): DDC classification:
  • 91-013943
In: Burnett, P.A. (ed.). World perspectives on barley yellow dwarf. Proceedings. Mexico, DF (Mexico). CIMMYT. 1990. p. 415-42091-013943Summary: The enzyme-linked immunosorbent assay (ELISA) technique was used with a local isolate of the PAV serotype to screen wild grasses for resistance to barley yellow dwarf virus (BYDV). Thirteen species of grass showed resistance in that they could maintain low levels of the virus for one month after inoculation. The F1 hybrids with wheat (Triticum aestivum L.) showed good resistance. Particularly promising was a partial amphiploid from China called Zhong 4, which showed very low levels of PAV in ELISA. Probes with various ribosomal spacer sequences and 5S ribosomal sequences of DNA isolated from Zhong 4 suggested that some alien chromosomes present
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The enzyme-linked immunosorbent assay (ELISA) technique was used with a local isolate of the PAV serotype to screen wild grasses for resistance to barley yellow dwarf virus (BYDV). Thirteen species of grass showed resistance in that they could maintain low levels of the virus for one month after inoculation. The F1 hybrids with wheat (Triticum aestivum L.) showed good resistance. Particularly promising was a partial amphiploid from China called Zhong 4, which showed very low levels of PAV in ELISA. Probes with various ribosomal spacer sequences and 5S ribosomal sequences of DNA isolated from Zhong 4 suggested that some alien chromosomes present

English

World Perspectives on Barley Yellow Dwarf International Workshop. Udine (Italy). 6-11 Jul 1987 CIMMYT, Ap. 6-641, 06600 Mexico, D.F. - Mexico|COMOD

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