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The use of cloned hybridization probes to detect barley yellow dwarf virus infection in tetraploid wheat germplasm

By: Pace, C. de | World Perspectives on Barley Yellow Dwarf International Workshop Udine (Italy) 6-11 Jul 1987.
Contributor(s): Casa, R [coaut.] | Delre, V [coaut.] | Porceddu, E [coaut.].
Material type: materialTypeLabelBookAnalytics: Show analyticsPublisher: 1990ISBN: 968-6127-39.Subject(s): Acids | Biological properties | Breeding methods | Cell structure | Chromosome number | Disease transmission | Gramineae | Luteoviruses | Natural resources | Nucleic acids | Nucleic compounds | Organic acids | Pathogenesis | Plant diseases | Plant viruses | Polyploidy | Progeny | Pure lines | Viruses AGROVOC | Genetic resources | TriticumDDC classification: 91-013967 In: Burnett, P.A. (ed.). World perspectives on barley yellow dwarf. Proceedings. Mexico, DF (Mexico). CIMMYT. 1990. p. 421-42891-013967Summary: A PAV-like isolate of barley yellow dwarf virus (BYDV) was used in controlled inoculation experiments in the greenhouse to determine the intensity of the autoradiographic signal from positive Northern dot-blot hybridization of leaf sample extract of infected and uninfected plants of tetraploid wheat (Triticum turgidum L.). Radioactive probes gave more consistent results than biotin probes. The proportion of entries with reduced virus content in collections from Ethiopia, Iran, Iraq, and Turkey were 24.3, 28.6, 8.7 and 10.5 %, respectively. No other differential symptoms were expressed on the infested plants in the greenhouse. Therefore, the furtherCollection: AGRIS Collection
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AGRIS Collection 91-013967 (Browse shelf) 1 Available 91-013967
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A PAV-like isolate of barley yellow dwarf virus (BYDV) was used in controlled inoculation experiments in the greenhouse to determine the intensity of the autoradiographic signal from positive Northern dot-blot hybridization of leaf sample extract of infected and uninfected plants of tetraploid wheat (Triticum turgidum L.). Radioactive probes gave more consistent results than biotin probes. The proportion of entries with reduced virus content in collections from Ethiopia, Iran, Iraq, and Turkey were 24.3, 28.6, 8.7 and 10.5 %, respectively. No other differential symptoms were expressed on the infested plants in the greenhouse. Therefore, the further

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World Perspectives on Barley Yellow Dwarf International Workshop. Udine (Italy). 6-11 Jul 1987 CIMMYT, Ap. 6-641, 06600 Mexico, D.F. - Mexico|COMOD

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