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PAV RNA concentration differences among field resistant and susceptible wheat cultivars

By: Lorens, G.F | World Perspectives on Barley Yellow Dwarf International Workshop Udine (Italy) 6-11 Jul 1987.
Contributor(s): Falk, B.W [coaut.] | Qualset, C.O [coaut.].
Material type: materialTypeLabelBookAnalytics: Show analyticsPublisher: 1990ISBN: 968-6127-39.Subject(s): Acids | Biological competition | Gramineae | Nucleic acids | Nucleic compounds | Organic acids | Parasitism | Plant diseases | Resistance to injurious factors | Triticum | Plant breeding AGROVOCDDC classification: 91-011675 In: Burnett, P.A. (ed.). World perspectives on barley yellow dwarf. Proceedings. Mexico, DF (Mexico). CIMMYT. 1990. p. 154-16091-011675Summary: Development of wheat cultivars (Triticum aestivum L.) resistant to barley yellow dwarf virus (BYDV) has often relied on visual scoring of symptoms under field conditions. To better understand the relationship of BYDV and host reaction, northern dot blot analysis was used to determine if four visually resistant cultivars had lower viral RNA concentrations relative to two susceptible cultivars. Plants were infected with the PAV isolate and grown in a growth chamber. Crude plant extracts were blotted on nitrocellulose and hybridized with 32P labeled probe of the pPA8 (a PAV clone provided by Wayne Gerlach of CSIRO). A dilution series of purified plasmidCollection: AGRIS Collection
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AGRIS Collection 91-011675 (Browse shelf) 1 Available 91-011675
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Development of wheat cultivars (Triticum aestivum L.) resistant to barley yellow dwarf virus (BYDV) has often relied on visual scoring of symptoms under field conditions. To better understand the relationship of BYDV and host reaction, northern dot blot analysis was used to determine if four visually resistant cultivars had lower viral RNA concentrations relative to two susceptible cultivars. Plants were infected with the PAV isolate and grown in a growth chamber. Crude plant extracts were blotted on nitrocellulose and hybridized with 32P labeled probe of the pPA8 (a PAV clone provided by Wayne Gerlach of CSIRO). A dilution series of purified plasmid

English

World Perspectives on Barley Yellow Dwarf International Workshop. Udine (Italy). 6-11 Jul 1987 CIMMYT, Ap. 6-641, 06600 Mexico, D.F. - Mexico|COMOD

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