Knowledge Center Catalog

Local cover image
Local cover image

Association of a lipoxygenase locus, Lpx-B1 , with variation in lipoxygenase activity in durum wheat seeds

By: Contributor(s): Material type: ArticleArticleLanguage: English Publication details: USA : CSSA : Wiley, 2002.ISSN:
  • 1435-0653 (Online)
  • 0011-183X
Subject(s): Online resources: In: Crop Science v. 42, no.5, p. 1695-1700632181Summary: Lipoxygenases constitute a family of enzymes that catalyze the breakdown of lipids, resulting in products that may have undesirable effects. These enzymes can affect pasta color and cause off-flavors. The purpose of this study was to map the loci associated with lipoxygenase activity in seeds of a durum wheat (Triticum turgidum ssp. durum) population to determine if lipoxygenase activity is associated with flour color. Seed of the parents, Jennah Khetifa and Cham 1, and the recombinant inbred population consisting of 113 progeny lines were used to assay lipoxygenase activity and flour color. A saturated molecular-marker linkage map for this population was previously constructed. Allele specific primers targeting the wheat homolog to barley LoxA were designed, and a fragment length polymorphism resulting from a miniature inverted-repeat transposable element (MITE) insertion in an intron of the durum wheat LoxA homolog allowed mapping of the locus to chromosome 4BS. To measure lipoxygenase activity, spectrophotometric changes were recorded. Cham 1 had a change in absorbance of 0.284 units of absorbance over 1 min compared with 0.752 units for Jennah Khetifa. Quantitative trait locus analysis of these data indicated that most of the lipoxygenase activity was associated with the wheat lipoxygenase gene (Lpx) on chromosome 4BS. A quantitative trait locus for vitreous appearance of seeds also was associated with this locus. Flour color was not correlated with lipoxygenase activity in this population. With this knowledge, marker-assisted selection can be used to select genotypes with lower lipoxygenase activity in durum wheat populations.
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Collection Call number Copy number Status Date due Barcode Item holds
Article CIMMYT Knowledge Center: John Woolston Library CIMMYT Staff Publications Collection CIS-3675 (Browse shelf(Opens below)) 1 Available 632181
Total holds: 0

Peer review

Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0011-183X

Lipoxygenases constitute a family of enzymes that catalyze the breakdown of lipids, resulting in products that may have undesirable effects. These enzymes can affect pasta color and cause off-flavors. The purpose of this study was to map the loci associated with lipoxygenase activity in seeds of a durum wheat (Triticum turgidum ssp. durum) population to determine if lipoxygenase activity is associated with flour color. Seed of the parents, Jennah Khetifa and Cham 1, and the recombinant inbred population consisting of 113 progeny lines were used to assay lipoxygenase activity and flour color. A saturated molecular-marker linkage map for this population was previously constructed. Allele specific primers targeting the wheat homolog to barley LoxA were designed, and a fragment length polymorphism resulting from a miniature inverted-repeat transposable element (MITE) insertion in an intron of the durum wheat LoxA homolog allowed mapping of the locus to chromosome 4BS. To measure lipoxygenase activity, spectrophotometric changes were recorded. Cham 1 had a change in absorbance of 0.284 units of absorbance over 1 min compared with 0.752 units for Jennah Khetifa. Quantitative trait locus analysis of these data indicated that most of the lipoxygenase activity was associated with the wheat lipoxygenase gene (Lpx) on chromosome 4BS. A quantitative trait locus for vitreous appearance of seeds also was associated with this locus. Flour color was not correlated with lipoxygenase activity in this population. With this knowledge, marker-assisted selection can be used to select genotypes with lower lipoxygenase activity in durum wheat populations.

Text in English

0307|Crop Science Society of America (CSSA)|AL-Wheat Program

Click on an image to view it in the image viewer

Local cover image

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org