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Direct isolation of cDNA sequences from specific chromosomal regions of the tomato genome by the differential display technique

By: Hannappel, U.
Contributor(s): Balzer, H.J [coaut.] | Ganal, M.W [coaut.].
Material type: materialTypeLabelArticlePublisher: 1995ISSN: 0026-8925.Subject(s): Acids | Cell structure | Chromosomes | Cytogenetics | Genomes | Lycopersicon | Nucleic acids | Nucleic compounds | Nucleus | Organic acids | Plant diseases | Plant genetics and breeding | Plant viruses | Resistance to injurious factors | Solanaceae | Tobamoviruses | Viruses AGROVOC | Genetics AGROVOCDDC classification: 97-089713 In: Molecular and General Genetics (Germany). (1995). v. 249(1) p. 19-24Summary: The differential display technique was originally developed for the isolation of differentially expressed genes from eukaryotic tissues. This technique for the isolation of cDNA markers from specific regions of the tomato genome was adapted. For this purpose, differential display was performed on RNA extracted from leaf tissue of nearly isogenic lines for the Tm-2a gene of tomato. On average, one out of 20 primer combinations resulted in a polymorphism at the cDNA level. When used as hybridization probes, all of these cDNA fragments were single or low copy and all of them were polymorphic on Southern hybridizations using DNA from the isogenic lines. Genetic mapping revealed in each case at least one locus in the introgressed segment on chromosome 9 of tomato. Thus, this technique might provide a way for the direct isolation of transcribed sequences from specific regions of any animal or plant genome for which such lines existCollection: AGRIS Collection
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Article CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

AGRIS Collection 97-089713 (Browse shelf) Available
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3 ill., 1 graph; 22 ref. Summary (En)

The differential display technique was originally developed for the isolation of differentially expressed genes from eukaryotic tissues. This technique for the isolation of cDNA markers from specific regions of the tomato genome was adapted. For this purpose, differential display was performed on RNA extracted from leaf tissue of nearly isogenic lines for the Tm-2a gene of tomato. On average, one out of 20 primer combinations resulted in a polymorphism at the cDNA level. When used as hybridization probes, all of these cDNA fragments were single or low copy and all of them were polymorphic on Southern hybridizations using DNA from the isogenic lines. Genetic mapping revealed in each case at least one locus in the introgressed segment on chromosome 9 of tomato. Thus, this technique might provide a way for the direct isolation of transcribed sequences from specific regions of any animal or plant genome for which such lines exist

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