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Plant regeneration from inflorescence cultures of hexaploid triticale

By: Contributor(s): Material type: ArticleArticlePublication details: 1982Subject(s): DDC classification:
  • 82-810568
In: Plant Science Letters (Netherlands). (Mar 1982). v. 24(3) p. 275-280Summary: A procedure for achieving plant regeneration from inflorescence-derived callus cultures of hexaploid triticale was developed. Genotypic differences in the frequency of totipotent callus formation were evident. Calli initiated on agar had a higher morphogenic capacity than those initiated on liquid medium. No growth regulator regimes enhanced the morphogenic response, except for root induction which was stimulated by gibberellic acid (GA(,3)) or triiodobenzoic acid (TIBA). Although the frequency of totipotent callus induction was low, large numbers of plants could be obtained from individual calli. The regenerated plants were generally shorter and less vigorous than the original donor material, but the majority of those examined had the expected euploid chromosome number indicating that their altered morphology had a physiological rather than a genetic basis
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16 ref.; Summary (En)

A procedure for achieving plant regeneration from inflorescence-derived callus cultures of hexaploid triticale was developed. Genotypic differences in the frequency of totipotent callus formation were evident. Calli initiated on agar had a higher morphogenic capacity than those initiated on liquid medium. No growth regulator regimes enhanced the morphogenic response, except for root induction which was stimulated by gibberellic acid (GA(,3)) or triiodobenzoic acid (TIBA). Although the frequency of totipotent callus induction was low, large numbers of plants could be obtained from individual calli. The regenerated plants were generally shorter and less vigorous than the original donor material, but the majority of those examined had the expected euploid chromosome number indicating that their altered morphology had a physiological rather than a genetic basis

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