In vitro uptake and incorporation of radiosotopes into proteins of wheat endosperm

By:

Graham, J.S.D

Contributor(s):

Material type: Article

ArticleLanguage: English Publication details: 1964. Australia : CSIRO,ISSN:

0004-9417

Subject(s):

In: Australian Journal of Biological Sciences v. 17, no. 1, p. 102-114Summary: A procedure is described for the quantitative i'301ation of the protein bodies from developing wheat endosperm. The changes with time in specific a.ctivity of the storage proteins (present in the protein bodies) and of the soluble proteins (present in the high-speed supernatant fraction) have been followed in "pulse"labelling experiments. Excised wheat heads were exposed to [35SJsulphate for periods of 5 min to 1 hr; dilution of the 35S-radioactivity within the wheat head before the incorporation of radioactivity into the endosperm proteins resulted in a constant ratio of the specific activity of the soluble proteins to that of the storage proteinfl.

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Item type	Current library	Collection	Call number	Status	Date due	Barcode	Item holds
Article	CIMMYT Knowledge Center: John Woolston Library	Reprints Collection	REP-1276 (Browse shelf(Opens below))	Available

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A procedure is described for the quantitative i'301ation of the protein bodies from developing wheat endosperm. The changes with time in specific a.ctivity of the storage proteins (present in the protein bodies) and of the soluble proteins (present in the high-speed supernatant fraction) have been followed in "pulse"labelling experiments. Excised wheat heads were exposed to [35SJsulphate for periods of 5 min to 1 hr; dilution of the 35S-radioactivity within the wheat head before the incorporation of radioactivity into the endosperm proteins resulted in a constant ratio of the specific activity of the soluble proteins to that of the storage proteinfl.

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In vitro uptake and incorporation of radiosotopes into proteins of wheat endosperm

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International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org

Knowledge Center Catalog

In vitro uptake and incorporation of radiosotopes into proteins of wheat endosperm

Browsing CIMMYT Knowledge Center: John Woolston Library shelves, Collection: Reprints Collection Close shelf browser (Hides shelf browser)

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021. Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237. If you have any question, please contact us at CIMMYT-Knowledge-Center@cgiar.org

International Maize and Wheat Improvement Center (CIMMYT) © Copyright 2021.
Carretera México-Veracruz. Km. 45, El Batán, Texcoco, México, C.P. 56237.
If you have any question, please contact us at
CIMMYT-Knowledge-Center@cgiar.org