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A flotation method for obtaining spores of helminthosporium sativum from soil

By: Contributor(s): Material type: ArticleArticleLanguage: English Publication details: 1955. Canada : National Research Council of Canada,ISSN:
  • 1916-2790
  • 1916-2804 (Online)
Subject(s): In: Canadian Journal of Botany v. 33, no. 4, p. 298-303Summary: This paper describes a flotation method by means of which spores of Helminthosporium sativum may be recovered from soil. Soil mixed with a small amount of mineral oil is shaken up in water. The emulsion which collects on the surface of the water contains most of the spores originally in the soil. Approximate spore numbers are determined by microscopic examination of aliquots of the emulsion. The technique is also well adapted for obtaining spores from soil for viability studies. It may be useful in comparing the effects of cultural and chemical treatments of soils on spore populations under field conditions.
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Article CIMMYT Knowledge Center: John Woolston Library Reprints Collection REP-681 (Browse shelf(Opens below)) Available
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This paper describes a flotation method by means of which spores of Helminthosporium sativum may be recovered from soil. Soil mixed with a small amount of mineral oil is shaken up in water. The emulsion which collects on the surface of the water contains most of the spores originally in the soil. Approximate spore numbers are determined by microscopic examination of aliquots of the emulsion. The technique is also well adapted for obtaining spores from soil for viability studies. It may be useful in comparing the effects of cultural and chemical treatments of soils on spore populations under field conditions.

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