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Type 2 callus production and plant regeneration in tropical maize genotypes

By: Carvalho, C.H.S.
Contributor(s): Abreu, L.L [coaut.] | Bohorova, N.E [coaut.] | Bordallo, P.N [coaut.] | Bressan, W [coaut.] | Paiva, E [coaut.] | Valicente, F.H [coaut.].
Material type: materialTypeLabelArticlePublisher: 1997Subject(s): Callus | Culture techniques | Dicamba | Maize | Tissue culture | Tropical zones | Vegetative propagation | Tropical maize CIMMYT | Zea mays AGROVOC In: Plant Cell Reports v. 17, p. 73-76627290Summary: A total of 113 maize inbreds adapted to tropical conditions were evaluated for their tissue culture response. Additionally, four media combinations of 15 or 30 mu M dicamba with or without 88 mu M AgNO3 were used to study the effect of dicamba and AgNO3 on type II callus production and plant regeneration from 42 of the inbred lines. Inbreds 48, 389 and 1345 of the populations BR 105, BR 112, and Catete, respectively, showed a high capacity for type II callus production and plant regeneration. The production of type II calli increased significantly when the concentration of dicamba was changed from 15 to 30 mu M and when AgNO3 was added to the medium. A synergistic effect between 88 mu M AgNO3 and 30 mu M dicamba (CM-30Ag medium) was observed, leading to additional production of type II callus. Medium CM-30Ag allowed the best tissue culture performance and plant regeneration capacityCollection: CIMMYT Staff Publications Collection
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Article CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

CIMMYT Staff Publications Collection CIS-2248 (Browse shelf) 1 Available 627290
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A total of 113 maize inbreds adapted to tropical conditions were evaluated for their tissue culture response. Additionally, four media combinations of 15 or 30 mu M dicamba with or without 88 mu M AgNO3 were used to study the effect of dicamba and AgNO3 on type II callus production and plant regeneration from 42 of the inbred lines. Inbreds 48, 389 and 1345 of the populations BR 105, BR 112, and Catete, respectively, showed a high capacity for type II callus production and plant regeneration. The production of type II calli increased significantly when the concentration of dicamba was changed from 15 to 30 mu M and when AgNO3 was added to the medium. A synergistic effect between 88 mu M AgNO3 and 30 mu M dicamba (CM-30Ag medium) was observed, leading to additional production of type II callus. Medium CM-30Ag allowed the best tissue culture performance and plant regeneration capacity

English

fer|Springer|9803|EE|anterior|R97-98ANALY|fernando2000|FINAL9798|3

CIMMYT Staff Publications Collection

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