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Determination of amino acids in wheat and barley by near-infrared reflectance spectroscopy

By: Contributor(s): Material type: ArticleArticlePublication details: 1984ISSN:
  • 0022-1147
Subject(s): DDC classification:
  • 85-009059|85-039937
In: Journal of Food Science (USA). (Jan-Feb 1984). v. 49(1) p. 17-20Summary: A study evaluated the utility of 2 research near-infrared reflectance (NIR) spectrometers and a commercial NIR spectrometer for the rapid screening analysis of 4 "limiting" amino acids in wheat, and up to 14 amino acids in barley. Spectral signals were recorded on 1 computerized research NIR spectrometer as log 1/R and transformed to the second-derivative of the signals for data analysis. This approach led to acceptable accuracies for the 4 wheat amino acids examined. For barley, high accuracy analysis was obtained for 13 amino acids using the other 2 NIR spectrometers with a first-derivative analysis method. NIR spectroscopy may be useful in amino acid analysis in plant-breeding studies, and in the food and feed industries for food analysis and protein quality evaluation. (wz)
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A study evaluated the utility of 2 research near-infrared reflectance (NIR) spectrometers and a commercial NIR spectrometer for the rapid screening analysis of 4 "limiting" amino acids in wheat, and up to 14 amino acids in barley. Spectral signals were recorded on 1 computerized research NIR spectrometer as log 1/R and transformed to the second-derivative of the signals for data analysis. This approach led to acceptable accuracies for the 4 wheat amino acids examined. For barley, high accuracy analysis was obtained for 13 amino acids using the other 2 NIR spectrometers with a first-derivative analysis method. NIR spectroscopy may be useful in amino acid analysis in plant-breeding studies, and in the food and feed industries for food analysis and protein quality evaluation. (wz)

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