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Biochemical and molecular diagnostics of Thinopyrum bessarabicum chromosomes in Triticum aestivum germplasm

By: William, M.D.H.M.
Contributor(s): Mujeeb-Kazi, A [coaut.].
Material type: materialTypeLabelArticlePublisher: 1995ISSN: 1432-2242 (Revista en electrónico).Subject(s): Agropyron | Genetic maps | Hybridization | Introgression | Salt tolerance | Triticum aestivumOnline resources: Access only for CIMMYT Staff In: Theoretical and Applied Genetics v. 90, no. 7-8, p. 952-956999416Summary: Thinopyrum bessarabicum [Elymus farctus subsp. bessarabicus] (2n = 2x = 14, JJ) is a self-fertile, salt-tolerant grass species, and its hybridization with Triticum aestivum to achieve the transfer of these attributes has been promoted. For the detection of alien introgression, development of diagnostic markers of T. bessarabicum chromosomes in the wheat background has emerged as an important aspect in this intergeneric hybridization programme. Six proteins/isozymes/high-molecular-weight glutenins, including superoxide dismutase, grain esterase, beta -amylase, glutamate oxaloacetate transaminase [aspartate aminotransferase] and alpha -amylase, were identified as positive markers for detecting the presence of T. bessarabicum chromosomes in the advanced backcross derivatives of T. aestivum/T. bessarabicum//n*T. aestivum. Fluorescent in situ hybridization further enabled the detection of complete and translocated arms of T. bessarabicum chromosomes in the T. aestivum background. These diagnostic markers served for tentatively characterizing a distinct set of T. bessarabicum disomic additions to wheat (2n = 44) and have facilitated establishing the homoeology of these added chromosomes
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Article CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

CIMMYT Staff Publications Collection Look under journal title (Browse shelf) 1 Available 999416
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Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752

Thinopyrum bessarabicum [Elymus farctus subsp. bessarabicus] (2n = 2x = 14, JJ) is a self-fertile, salt-tolerant grass species, and its hybridization with Triticum aestivum to achieve the transfer of these attributes has been promoted. For the detection of alien introgression, development of diagnostic markers of T. bessarabicum chromosomes in the wheat background has emerged as an important aspect in this intergeneric hybridization programme. Six proteins/isozymes/high-molecular-weight glutenins, including superoxide dismutase, grain esterase, beta -amylase, glutamate oxaloacetate transaminase [aspartate aminotransferase] and alpha -amylase, were identified as positive markers for detecting the presence of T. bessarabicum chromosomes in the advanced backcross derivatives of T. aestivum/T. bessarabicum//n*T. aestivum. Fluorescent in situ hybridization further enabled the detection of complete and translocated arms of T. bessarabicum chromosomes in the T. aestivum background. These diagnostic markers served for tentatively characterizing a distinct set of T. bessarabicum disomic additions to wheat (2n = 44) and have facilitated establishing the homoeology of these added chromosomes

English

WP|Springer|R95ANALY|1

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