Visualization of Secale cereale DNA in wheat germ plasm by fluorescent in situ hybridization
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ArticleLanguage: En Publication details: 1995ISSN: - 1432-2242 (Revista en electrónico)
| Item type | Current library | Collection | Call number | Copy number | Status | Date due | Barcode | Item holds | |
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| Article | CIMMYT Knowledge Center: John Woolston Library | CIMMYT Staff Publications Collection | Look under journal title (Browse shelf(Opens below)) | 1 | Available | 999465 |
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Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752
Homozygous wheat/rye (1BL/1RS or 1AS/1RL) translocation lines have significantly contributed to wheat production, and several other wheat/rye translocation lines show a potential promise against biotic and abiotic stresses. Detecting the presence of rye at the chromosome level is feasible by C-banding and isozyme protocols, but the diagnostic strength of genomic in situ hybridization for eventually analysing smaller DNA introgressions has greater significance. Non-radioactive in situ hybridization using labelled total genomic DNA of alien species was successfully utilized with the unlabelled blocking DNA of T. aestivum and T. durum. By this method rye contributions to the translocations 1Bll/1RS, 1AL/1RS, 5AS/5RL and 6BS/6RL could be identified. Differential labelling has further enabled the detection of rye and Thinopyrum bessarabicum [Elymus bessarabicus] chromosomes in a trigeneric hybrid of Triticum aestivum/T. bessarabicum/Secale cereale
English
WP|Springer|R95ANALY|1