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Development of DNA markers based on randomly amplified polymorphic sequences in a Triticum aestivum L. x Thinopyrum bessarabicum amphiploid

By: William, M.D.H.M.
Contributor(s): Gray, L [coaut.] | Mujeeb-Kazi, A [coaut.] | Rayburn, A.L [coaut.].
Material type: materialTypeLabelArticlePublisher: 1994Subject(s): Chromosome manipulation | Crossbreeding | DNA | Genetic engineering | Hybridization | Polymorphism | Triticum aestivumOnline resources: Access only for CIMMYT Staff In: Journal of Genetics and Breeding v. 48, no. 1, p. 1-6999556Summary: Randomly amplified polymorphic DNA (RAPD) markers were developed using the polymerase chain reaction (PCR) for the amphiploid of Triticum aestivum cv. Chinese Spring and Thinopyrum bessarabicum [Elymus farctus subsp. bessarabicus]. Of the 26 arbitrary oligonucleotide decamer primers studied, 13 produced easily detectable polymorphisms; 7 of these are reported in detail. There was no polymorphism among DNA extracts from individual seedlings of Chinese Spring. The amplification patterns generated with primers for the two plant species were repeatable. The marker bands specific to T. bessarabicum may be helpful in identifying its chromatin in the wheat background when chromosome additions, substitutions, translocations or subtle chromosomal interchanges are produced
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Article CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

CIMMYT Staff Publications Collection Look under journal title (Browse shelf) 1 Available 999556
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Peer-review: No - Open Access: No

Randomly amplified polymorphic DNA (RAPD) markers were developed using the polymerase chain reaction (PCR) for the amphiploid of Triticum aestivum cv. Chinese Spring and Thinopyrum bessarabicum [Elymus farctus subsp. bessarabicus]. Of the 26 arbitrary oligonucleotide decamer primers studied, 13 produced easily detectable polymorphisms; 7 of these are reported in detail. There was no polymorphism among DNA extracts from individual seedlings of Chinese Spring. The amplification patterns generated with primers for the two plant species were repeatable. The marker bands specific to T. bessarabicum may be helpful in identifying its chromatin in the wheat background when chromosome additions, substitutions, translocations or subtle chromosomal interchanges are produced

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