Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0032-0889

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Photoplhosphorylation by isolate(i chlioroplasts has been reportedl and studied in several species (21), but no publislhed evidence of the reaction by maize chlioroplasts coul(l )e foundl. In a stu(ly of this reaction by maize chlioroplasts initial experiments using existing isolation techniques (2, 12) failed to give active chloroplasts. One previous report of a smiliar inability to demonstrate photophosphorylation has been miadle by Heber (11) who was unable to obtain chloroplasts capable of photophosphorylating from Vicia faba. In this case a substance whiclh inhibited the reaction by spinach chloroplasts was found in the bean cell sap. The aims of this study w,ere to determine if maize leaf tissue contained a component that inhibite(d chloroplast activity and to (levise nmethods of extraction that would permit the isolation of chloroplasts from mnaize leaf tissue which would be capable of carrying out the Hill reaction and cyclic and(l noncyclic photophosphorylation.

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