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Improved anther culture method for obtaining direct regeneration in wheat (Triticum aestivum L.)

By: Material type: ArticleArticleLanguage: En Publication details: 1992ISSN:
  • 0394-9257
Other title:
  • Un metodo migliorato di coltura delle antere per ottenere la rigenerazione diretta nel grano (Triticum aestivum L.)
Subject(s): DDC classification:
  • 93-096101
In: Journal of Genetics and Breeding v. 46, no. 1, p. 99-10293-096101Summary: Anther culture response of two wheat cultivars was tested with various barley-starch and maltose containing media. A considerable increase in direct plant regeneration (i.e. without transfer to a regeneration media) was obtained when medium was supplemented with silver nitrate. Up to 18 haploid plants per 100 anthers were directly produced. By using a single-step procedure, decreased costs in wheat haploid production can be effectively achieved
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2 tables; 14 ref. Summary (En)

Peer-review: No - Open Access: No

Anther culture response of two wheat cultivars was tested with various barley-starch and maltose containing media. A considerable increase in direct plant regeneration (i.e. without transfer to a regeneration media) was obtained when medium was supplemented with silver nitrate. Up to 18 haploid plants per 100 anthers were directly produced. By using a single-step procedure, decreased costs in wheat haploid production can be effectively achieved

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