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Amino acid transport in suspension-cultured plant cells, 4: biphasic saturable uptake kinetics of L-leucine in isolates from six Nicotiana tabacum plants

By: McDaniel, C.N.
Contributor(s): Blackman, M.S [coaut.] | Lyons, R.A [coaut.].
Material type: materialTypeLabelArticlePublisher: 1981Subject(s): Meristems | Tobacco nicotiana sppDDC classification: 82-766860 In: Plant Science Letters v. 23, no. 1, p. 17-2382-766860Summary: Kinetic analyses of L-leucine uptake were made on suspension-cultured cells initially isolated from pith tissue of 6 Nicotiana tabacum cv. Wisconsin 38 plants; D cells from a diploid plant, H1 and H2 cells from two haploid plants, Z cells from another diploid plant, and Z2 and Z4 cells from two plants regenerated from Z cells. Uptake rates were measured at 12 L-Leu concentrations over a concentration range 0.01-2.0 mM in a homogeneous population of cells. Data from several populations of cells were pooled and kinetic analyses of the uptake rates indicated that uptake can be described in all 6 cell lines by two saturable phases. The lowerCollection: AGRIS Collection
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Article CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

AGRIS Collection 82-766860 (Browse shelf) 1 Available 82-766860
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16 ref.; Summary (En)

Kinetic analyses of L-leucine uptake were made on suspension-cultured cells initially isolated from pith tissue of 6 Nicotiana tabacum cv. Wisconsin 38 plants; D cells from a diploid plant, H1 and H2 cells from two haploid plants, Z cells from another diploid plant, and Z2 and Z4 cells from two plants regenerated from Z cells. Uptake rates were measured at 12 L-Leu concentrations over a concentration range 0.01-2.0 mM in a homogeneous population of cells. Data from several populations of cells were pooled and kinetic analyses of the uptake rates indicated that uptake can be described in all 6 cell lines by two saturable phases. The lower

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