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Characteristics of Hiproly barley, 1: isolation and characterisation of two water-soluble high-lysine proteins [antibody production, antigenicity, molecular weight, isoelectric point, albumin]

By: Jonassen, I.
Material type: materialTypeLabelArticlePublisher: 1980ISSN: 0105-1938.Subject(s): Barley hordeum spp | Plant breeding AGROVOCDDC classification: 81-596334 In: Carlsberg Research Communications v. 45, no. 1, p. 47-5881-596334Summary: The water-soluble albumin of the high-lysine barley called Hiproly was fractionated by means of ion exchange chromatography and gel filtration. Three high-lysine fractions were isolated, and from the chromatographic profiles it was deduced that two of these contribute prominently to the overall high lysine content of Hiproly albumin. The two polypeptides were purified, and antisera raised against them. Immunological cross reaction was observed suggesting that the smaller polypeptide with a molecular weight of 8,100 dalton is a fragment of the larger one with a molecular weight of 9,100 dalton. The fragment contains 6 and the larger polypeptide 8 lysineCollection: AGRIS Collection
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Article CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

AGRIS Collection 81-596334 (Browse shelf) 1 Available 81-596334
Total holds: 0

9 ill., 3 tables; 23 ref. Summary (En)

The water-soluble albumin of the high-lysine barley called Hiproly was fractionated by means of ion exchange chromatography and gel filtration. Three high-lysine fractions were isolated, and from the chromatographic profiles it was deduced that two of these contribute prominently to the overall high lysine content of Hiproly albumin. The two polypeptides were purified, and antisera raised against them. Immunological cross reaction was observed suggesting that the smaller polypeptide with a molecular weight of 8,100 dalton is a fragment of the larger one with a molecular weight of 9,100 dalton. The fragment contains 6 and the larger polypeptide 8 lysine

English

Carlsberg Lab., Copenhagen (Denmark)|COMOD

AGRIS Collection

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