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The determination of available lysine in barley using Tetrahymena pyriformis W

By: Contributor(s): Material type: ArticleArticleLanguage: En Publication details: 1980Subject(s): DDC classification:
  • 81-589049
 In: Animal Feed Science and Technology v. 5, no. 1, p. 33-4281-589049Summary: Attempts to use the microbiological procedure based on growth of Tetrahymena for the assay of available lysine in barley led to several technical problems. These were mainly due to interference by carbohydrate with the measurement of the growth response of the organism, either by cell counting or by optical density. The assay was modified to suit the measurement of available lysine in barley grains. Fine grinding of samples, predigestion with papain and selection of appropriate N-concentrations in the medium were found to be key factors in adapting the technique for barley. The availability of lysine in barley, as assessed by the modified
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11 ref.; Summary (En)

Attempts to use the microbiological procedure based on growth of Tetrahymena for the assay of available lysine in barley led to several technical problems. These were mainly due to interference by carbohydrate with the measurement of the growth response of the organism, either by cell counting or by optical density. The assay was modified to suit the measurement of available lysine in barley grains. Fine grinding of samples, predigestion with papain and selection of appropriate N-concentrations in the medium were found to be key factors in adapting the technique for barley. The availability of lysine in barley, as assessed by the modified

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