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Thinophyrum bessarabicum: Biochemical and cytological markers for the detection of genetic introgression in its hybrid derivatives with Triticum aestivum L.

By: William, M.D.H.M.
Contributor(s): Mujeeb-Kazi, A [coaut.].
Material type: materialTypeLabelArticlePublisher: 1993ISSN: 1432-2242 (Revista en electrónico).Subject(s): Cytology | Introgression | Salt tolerance | Triticum aestivum | Hybrids AGROVOCOnline resources: Access only for CIMMYT Staff In: Theoretical and Applied Genetics v. 86, no. 2, p. 365-370999850Summary: Thinopyrum bessarabicum [Elymus farctus subsp. bessarabicus] (2n = 2x = 14, JJ) with its unique property of salt tolerance provides a potential means for the transfer of this important and complex trait into cultivated wheat through intergeneric hybridization. To accomplish this, diagnostic markers for detecting the presence of T. bessarabicum chromosomes in a wheat background have to be established. The C-banded karyotype of T. bessarabicum distinctly identifies individual T. bessarabicum chromosomes and separates them from those of Triticum aestivum. Also, seven protein/isozymes, i.e., malate dehydrogenase, high-molecular-weight glutenin, superoxide dismutase, grain esterase, glutamate oxaloacetate transaminase [aspartate aminotransferase], beta -amylase and alpha -amylase, were identified as being positive markers specific to T. bessarabicum; these were also expressed in the T. aestivum/T. bessarabicum amphiploid. These diagnostic biochemical markers could be useful in detecting and establishing homoeology of T. bessarabicum chromosomes in T. aestivum/T. bessarabicum intergeneric hybrid derivatives
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Article CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

CIMMYT Staff Publications Collection Look under journal title (Browse shelf) 1 Available 999850
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Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0040-5752

Thinopyrum bessarabicum [Elymus farctus subsp. bessarabicus] (2n = 2x = 14, JJ) with its unique property of salt tolerance provides a potential means for the transfer of this important and complex trait into cultivated wheat through intergeneric hybridization. To accomplish this, diagnostic markers for detecting the presence of T. bessarabicum chromosomes in a wheat background have to be established. The C-banded karyotype of T. bessarabicum distinctly identifies individual T. bessarabicum chromosomes and separates them from those of Triticum aestivum. Also, seven protein/isozymes, i.e., malate dehydrogenase, high-molecular-weight glutenin, superoxide dismutase, grain esterase, glutamate oxaloacetate transaminase [aspartate aminotransferase], beta -amylase and alpha -amylase, were identified as being positive markers specific to T. bessarabicum; these were also expressed in the T. aestivum/T. bessarabicum amphiploid. These diagnostic biochemical markers could be useful in detecting and establishing homoeology of T. bessarabicum chromosomes in T. aestivum/T. bessarabicum intergeneric hybrid derivatives

English

R93ANALY|Springer|1

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