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Somatic embryogenesis and plant regeneration of tripsacum dactyloides L.

By: Contributor(s): Material type: ArticleArticleLanguage: English Publication details: Dordrecht (Netherlands) : Springer, 1991.ISSN:
  • 1573-5060 (Online)
  • 0014-2336
Subject(s): In: Euphytica v. 55, no. 2, p. 111-115619339, 620532Summary: This article reports the culture and plant regeneration of Tripsacum dactyloides. Mature embryos of Tripsacum dactyloides dactyloides were used to obtain embryogenic callus cultures. Currently, 180 normal plants have been regenerated from these cultures. Callus was initiated on MS medium supplemented with dicamba (10 μmol or 20 μmol) and sucrose (3% or 6%), and plants were regenerated on hormone free MS medium containing 2% sucrose. No significant differences were found in callus initiation frequency or in embryogenic response of cultures on the four combinations of sucrose and dicamba tested. The embryogenic cultures have been maintained for 9 months (12 subcultures) and have retained regeneration capacity. Plants regenerated from tissue culture of maize-by-Tripsacum hybrids could be useful in maize improvement.
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Holdings
Item type Current library Collection Call number Copy number Status Date due Barcode Item holds
Article CIMMYT Knowledge Center: John Woolston Library CIMMYT Staff Publications Collection CIS-1529 (Browse shelf(Opens below)) 1 Available 619339
Article CIMMYT Knowledge Center: John Woolston Library CIMMYT Staff Publications Collection CIS-1529 (Browse shelf(Opens below)) 2 Available 620532
Total holds: 0

Peer-review: Yes - Open Access: Yes|http://science.thomsonreuters.com/cgi-bin/jrnlst/jlresults.cgi?PC=MASTER&ISSN=0014-2336

Peer review

This article reports the culture and plant regeneration of Tripsacum dactyloides. Mature embryos of Tripsacum dactyloides dactyloides were used to obtain embryogenic callus cultures. Currently, 180 normal plants have been regenerated from these cultures. Callus was initiated on MS medium supplemented with dicamba (10 μmol or 20 μmol) and sucrose (3% or 6%), and plants were regenerated on hormone free MS medium containing 2% sucrose. No significant differences were found in callus initiation frequency or in embryogenic response of cultures on the four combinations of sucrose and dicamba tested. The embryogenic cultures have been maintained for 9 months (12 subcultures) and have retained regeneration capacity. Plants regenerated from tissue culture of maize-by-Tripsacum hybrids could be useful in maize improvement.

Text in English

MP|Springer|12219-R|3

CIMMYT Staff Publications Collection

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