Use of ELISA for measuring the extent of serological cross-reactivity between plant viruses
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Journal of Virological Methods v. 11, no. 3, p. 189-198617580Summary: The degree of antigenic relatedness between 2 plant viruses is commonly expressed by a serological differentiation index (SDI) which corresponds to the av. number of 2-fold dilution steps separating homologous from heterologous precipitin titres. Results obtained with several tobamo- and tombusviruses indicated that the indirect form of ELISA can also be used for calculating SDI values. This was achieved by comparing the antiserum dilutions that lead to the same absorbance measurements when homologous and heterologous viruses are assayed by ELISA. SDI values calculated from ELISA were similar to those obtained from precipitin tests. Because of its
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The degree of antigenic relatedness between 2 plant viruses is commonly expressed by a serological differentiation index (SDI) which corresponds to the av. number of 2-fold dilution steps separating homologous from heterologous precipitin titres. Results obtained with several tobamo- and tombusviruses indicated that the indirect form of ELISA can also be used for calculating SDI values. This was achieved by comparing the antiserum dilutions that lead to the same absorbance measurements when homologous and heterologous viruses are assayed by ELISA. SDI values calculated from ELISA were similar to those obtained from precipitin tests. Because of its
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