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Identification of a transposon-like insertion in a Glu1 allele of wheat

By: Harberd, N.P.
Contributor(s): Flavell, R.B [coaut.] | Thompson, R.D [coaut.].
Material type: materialTypeLabelArticlePublisher: 1987Subject(s): DNA | Genes | Loci AGROVOC | Peptides | Glutenins AGROVOC | Wheat AGROVOC In: Molecular and General Genetics v. 209, no. 2, p. 326-332615035Summary: Hexaploid wheat varieties carry a 'silent' Glu1y (glutenin) allele on chromosome 1A, no polypeptide being attributed to this locus. When 2 such alleles from cv. Cheyenne and cv. Chinese Spring were compared, the Chinese Spring allele was found to contain an 8 kbp insertion in the coding sequence, termed Wis2 (wheat insertion sequence 2). The insertion site is flanked by a 5 bp duplication. The 2 ends of Wis2 contain similar sequences over 500 bp long and its termini have almost the same short (9 or 10 bp) sequences but in opposite orientation. These terminal sequences are related to those of several 'retroposon'-type transposable elements found in otherCollection: Reprints Collection
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Article CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

Reprints Collection REP-4159 (Browse shelf) 1 Available 615035
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Illustrations, tables, references p. 331-332

Hexaploid wheat varieties carry a 'silent' Glu1y (glutenin) allele on chromosome 1A, no polypeptide being attributed to this locus. When 2 such alleles from cv. Cheyenne and cv. Chinese Spring were compared, the Chinese Spring allele was found to contain an 8 kbp insertion in the coding sequence, termed Wis2 (wheat insertion sequence 2). The insertion site is flanked by a 5 bp duplication. The 2 ends of Wis2 contain similar sequences over 500 bp long and its termini have almost the same short (9 or 10 bp) sequences but in opposite orientation. These terminal sequences are related to those of several 'retroposon'-type transposable elements found in other

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