Exponential amplification of recombinant-RNA hybridization probes
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BioTechnology v. 6, no. 10, p. 1197-1202615012Summary: Recombinant RNA molecules were synthesized that function both as hybridization probes and as templates for exponential amplification by Qbeta replicase. Each recombinant consists of a sequence specific for Plasmodium falciparum, embedded within the sequence of MDV-1 RNA, which is a natural template for Qbeta replicase. The probe sequence was inserted within a hairpin loop that occurs on the exterior of MDV-1 RNA. The recombinant RNAs hybridize specifically to complementary DNA, despite topological constraints on the probe domain, are replicated at the same rate as MDV-1 RNA, despite their additional length, and are able to serve as templates for the
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Recombinant RNA molecules were synthesized that function both as hybridization probes and as templates for exponential amplification by Qbeta replicase. Each recombinant consists of a sequence specific for Plasmodium falciparum, embedded within the sequence of MDV-1 RNA, which is a natural template for Qbeta replicase. The probe sequence was inserted within a hairpin loop that occurs on the exterior of MDV-1 RNA. The recombinant RNAs hybridize specifically to complementary DNA, despite topological constraints on the probe domain, are replicated at the same rate as MDV-1 RNA, despite their additional length, and are able to serve as templates for the
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