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Somaclonal variation - a new source of wheat germplasms

By: Contributor(s): Material type: ArticleArticleLanguage: En Publication details: 1988Subject(s): In: Utah Science v. 49, no. 3, p. 83-88615175Summary: Following culture of immature inflorescences and embryos of PCYT10, plants of 2 selfed somaclonal generations (SC1 and SC2) were raised in the greenhouse with the parent variety. Significant differences between parent and somaclones were observed in flag leaf size, plant height, number of tillers, ear length, awn length and number of grains/ear. Microscopic studies revealed these to be the result of gross chromosomal changes in some somaclonal plants. Isoenzyme analysis revealed that one SC1 family had a reduced endopeptidase band and segregation in the SC2 gave bands which were normal, of reduced intensity or missing, suggesting that a single
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Article CIMMYT Knowledge Center: John Woolston Library Reprints Collection REP-4577 (Browse shelf(Opens below)) 1 Available 615175
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Illustrations, literature cited p. 88

Following culture of immature inflorescences and embryos of PCYT10, plants of 2 selfed somaclonal generations (SC1 and SC2) were raised in the greenhouse with the parent variety. Significant differences between parent and somaclones were observed in flag leaf size, plant height, number of tillers, ear length, awn length and number of grains/ear. Microscopic studies revealed these to be the result of gross chromosomal changes in some somaclonal plants. Isoenzyme analysis revealed that one SC1 family had a reduced endopeptidase band and segregation in the SC2 gave bands which were normal, of reduced intensity or missing, suggesting that a single

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