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Somaclonal variation - a new source of wheat germplasms

By: Hashim, Z.N.
Contributor(s): Ahmed, S.U [coaut.] | Campbell, W.F [coaut.] | Carman, J.G [coaut.].
Material type: materialTypeLabelArticlePublisher: 1988Subject(s): Genetic engineering | Genetic variation | Germplasm | Isoenzymes | Tissue culture | Wheat AGROVOC | Plant breeding AGROVOC In: Utah Science v. 49, no. 3, p. 83-88615175Summary: Following culture of immature inflorescences and embryos of PCYT10, plants of 2 selfed somaclonal generations (SC1 and SC2) were raised in the greenhouse with the parent variety. Significant differences between parent and somaclones were observed in flag leaf size, plant height, number of tillers, ear length, awn length and number of grains/ear. Microscopic studies revealed these to be the result of gross chromosomal changes in some somaclonal plants. Isoenzyme analysis revealed that one SC1 family had a reduced endopeptidase band and segregation in the SC2 gave bands which were normal, of reduced intensity or missing, suggesting that a singleCollection: Reprints Collection
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Article CIMMYT Knowledge Center: John Woolston Library

Lic. Jose Juan Caballero Flores

 

Reprints Collection REP-4577 (Browse shelf) 1 Available 615175
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Illustrations, literature cited p. 88

Following culture of immature inflorescences and embryos of PCYT10, plants of 2 selfed somaclonal generations (SC1 and SC2) were raised in the greenhouse with the parent variety. Significant differences between parent and somaclones were observed in flag leaf size, plant height, number of tillers, ear length, awn length and number of grains/ear. Microscopic studies revealed these to be the result of gross chromosomal changes in some somaclonal plants. Isoenzyme analysis revealed that one SC1 family had a reduced endopeptidase band and segregation in the SC2 gave bands which were normal, of reduced intensity or missing, suggesting that a single

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