MARC details
| 000 -LEADER |
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| fixed length control field |
03088nam a22004217a 4500 |
| 001 - CONTROL NUMBER |
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| control field |
G94887 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-TxCIM |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20211006082355.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
121211s ||||f| 0 p|p||0|| | |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
MX-TxCIM |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
CIS-6278 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Li Liu |
| Affiliation |
International Triticeae Mapping Initiative 20; Wheat Genomics in China 2; Beijing (China); 1-5 Sep 2010 |
| 245 00 - TITLE STATEMENT |
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| Title |
Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Place of publication, distribution, etc. |
Beijing (China) |
| Name of publisher, distributor, etc. |
Chinese Academy of Agricultural Sciences : |
| Date of publication, distribution, etc. |
2010 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
p. 196 |
| 500 ## - GENERAL NOTE |
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| General note |
Abstract only |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
At the Glu-A3 locus, all seven alleles could be reliably identified by 2-DE and PCR. However, the alleles Glu-A3e and Glu-A3d could not be routinely distinguished from Glu-A3f and Glu-A3g, respectively, based on SDS-PAGE, and the allele Glu-A3a could not be differentiated from Glu-A3c by MALDI-TOF-MS. At the Glu-B3 locus, alleles Glu-B3a, Glu-B3b, Glu-B3c,Glu-B3g, Glu-B3h and Glu-B3j could be clearly identified by all four methods, whereas Glu-B3ab, Glu-B3ac, Glu-B3ad could only be identified by the 2-DE method. At the Glu-D3 locus, allelic identification was problematic for the electrophoresis based methods and PCR. MALDI-TOF-MS has the potential to reliably identify the Glu-D3 alleles. PCR is the simplest, most accurate, lowest cost, and therefore recommended method for identification of Glu-A3 and Glu-B3 alleles in breeding programs. A combination of methods was required to identify certain alleles, and would be especially useful when characterizing new alleles. A standard set of 30 cultivars for use in future studies was chosen to represent all LMW-GS allelic variants in the collection. Among them, Chinese Spring, Opata 85, Seri 82 and Pavon 76 were recommended as a core set for use in SDS-PAGE gels. Glu-D3c and Glu-D3e are the same allele. Two new alleles, namely, Glu-D3m in cultivar Darius, and Glu-D3n in Fengmai 27, were identified by 2-DE. Utilization of the suggested standard cultivar set, seed of which is available from the CIMMYT and INRA Clermont-Ferrand germplasm collections, should also promote information sharing in the identification of individual LMW-GS and thus provide useful information for quality improvement in common wheat. |
| 536 ## - FUNDING INFORMATION NOTE |
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| Text of note |
Global Wheat Program |
| 546 ## - LANGUAGE NOTE |
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| Language note |
English |
| 594 ## - STAFFID |
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| StaffID |
INT2411|INT0368 |
| 595 ## - COLLECTION |
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| Collection |
CIMMYT Staff Publications Collection |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Aili Wang, |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
1980 |
| Personal name |
Appels, R. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Branlard, G., |
| Relator term |
coaut. |
| 9 (RLIN) |
1976 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Hisashi Yoshida, |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Kolman, M.A., |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Lerner, S.E., |
| Relator term |
coaut. |
| 9 (RLIN) |
1977 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Linhai Wang, |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Rogers, W.J., |
| Relator term |
coaut. |
| 9 (RLIN) |
1975 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Tatsuya M Ikeda, |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Wujun Ma, |
| Relator term |
coaut. |
| 9 (RLIN) |
1979 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Xianchun Xia, |
| Relator term |
coaut. |
| 9 (RLIN) |
377 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Yueming Yan, |
| Relator term |
coaut. |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
He Zhonghu |
| Miscellaneous information |
Global Wheat Program |
| Field link and sequence number |
INT2411 |
| 9 (RLIN) |
838 |
| 700 1# - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Peña-Bautista, R.J. |
| Field link and sequence number |
INT0368 |
| Miscellaneous information |
Global Wheat Program |
| 9 (RLIN) |
645 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Conference proceedings |